論文 - 詳細
| RRC ID | 80913 |
|---|---|
| 著者 | Robert VJ, Caron M, Gely L, Adrait A, Pakulska V, Couté Y, Chevalier M, Riedel CG, Bedet C, Palladino F. |
| タイトル | SIN-3 acts in distinct complexes to regulate the germline transcriptional program in Caenorhabditis elegans. |
| ジャーナル | Development |
| Abstract |
The transcriptional co-regulator SIN3 influences gene expression through multiple interactions that include histone deacetylases. Haploinsufficiency and mutations in SIN3 are the underlying cause of Witteveen-Kolk syndrome and related intellectual disability and autism syndromes, emphasizing its key role in development. However, little is known about the diversity of its interactions and functions in developmental processes. Here, we show that loss of SIN-3, the single SIN3 homolog in Caenorhabditis elegans, results in maternal-effect sterility associated with de-regulation of the germline transcriptome, including de-silencing of X-linked genes. We identify at least two distinct SIN3 complexes containing specific histone deacetylases and show that they differentially contribute to fertility. Single-cell, single-molecule fluorescence in situ hybridization reveals that in sin-3 mutants the X chromosome becomes re-expressed prematurely and in a stochastic manner in individual germ cells, suggesting a role for SIN-3 in its silencing. Furthermore, we identify histone residues whose acetylation increases in the absence of SIN-3. Together, this work provides a powerful framework for the in vivo study of SIN3 and associated proteins. |
| 巻・号 | 150(21) |
| 公開日 | 2023-11-1 |
| DOI | 10.1242/dev.201755 |
| PII | 333417 |
| PMID | 37818613 |
| PMC | PMC10617626 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Germ Cells / metabolism Histone Deacetylases* / genetics Histone Deacetylases* / metabolism Histones / metabolism In Situ Hybridization, Fluorescence Sin3 Histone Deacetylase and Corepressor Complex* / genetics Sin3 Histone Deacetylase and Corepressor Complex* / metabolism X Chromosome / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1276 tm176 tm4223 tm6369 tm4233 |