RRC ID 80934
Author Wang R, Li J, Tian Y, Sun Y, Zhang Y, Liu M, Zhang R, Zhao L, Li Q, Meng X, Zhou J, Gao J.
Title The dynamic recruitment of LAB proteins senses meiotic chromosome axis differentiation in C. elegans.
Journal J Cell Biol
Abstract During meiosis, cohesin and meiosis-specific proteins organize chromatin into an axis-loop architecture, coordinating homologous synapsis, recombination, and ordered chromosome segregation. However, how the meiotic chromosome axis is assembled and differentiated with meiotic progression remains elusive. Here, we explore the dynamic recruitment of two long arms of the bivalent proteins, LAB-1 and LAB-2, in Caenorhabditis elegans. LAB proteins directly interact with the axis core HORMA complexes and weak interactions contribute to their recruitment. LAB proteins phase separate in vitro, and this capacity is promoted by HORMA complexes. During early prophase, synapsis oppositely regulates the axis enrichment of LAB proteins. After the pachytene exit, LAB proteins switch from a reciprocal localization pattern to a colocalization pattern, and the normal dynamic pattern of LAB proteins is altered in meiotic mutants. We propose that LAB recruitment senses axis differentiation, and phase separation of meiotic structures helps subdomain establishment and accurate segregation of the chromosomes.
Volume 223(2)
Published 2024-2-5
DOI 10.1083/jcb.202212035
PII 276431
PMID 38010234
PMC PMC10666650
MeSH Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromosomal Proteins, Non-Histone* / genetics Chromosomal Proteins, Non-Histone* / metabolism Chromosome Pairing / genetics Chromosome Segregation Chromosomes / genetics Chromosomes / metabolism Meiosis*
Resource
C.elegans tm3655 tm1791