RRC ID 80937
Author Saredi G, Carelli FN, Rolland SGM, Furlan G, Piquet S, Appert A, Sanchez-Pulido L, Price JL, Alcon P, Lampersberger L, Déclais AC, Ramakrishna NB, Toth R, Macartney T, Alabert C, Ponting CP, Polo SE, Miska EA, Gartner A, Ahringer J, Rouse J.
Title The histone chaperone SPT2 regulates chromatin structure and function in Metazoa.
Journal Nat Struct Mol Biol
Abstract Histone chaperones control nucleosome density and chromatin structure. In yeast, the H3-H4 chaperone Spt2 controls histone deposition at active genes but its roles in metazoan chromatin structure and organismal physiology are not known. Here we identify the Caenorhabditis elegans ortholog of SPT2 (CeSPT-2) and show that its ability to bind histones H3-H4 is important for germline development and transgenerational epigenetic gene silencing, and that spt-2 null mutants display signatures of a global stress response. Genome-wide profiling showed that CeSPT-2 binds to a range of highly expressed genes, and we find that spt-2 mutants have increased chromatin accessibility at a subset of these loci. We also show that SPT2 influences chromatin structure and controls the levels of soluble and chromatin-bound H3.3 in human cells. Our work reveals roles for SPT2 in controlling chromatin structure and function in Metazoa.
Volume 31(3)
Pages 523-535
Published 2024-3-1
DOI 10.1038/s41594-023-01204-3
PII 10.1038/s41594-023-01204-3
PMID 38238586
PMC PMC7615752
MeSH Animals Chromatin / metabolism DNA-Binding Proteins* / metabolism Histone Chaperones* / genetics Histone Chaperones* / metabolism Histones / metabolism Humans Nucleosomes / metabolism Saccharomyces cerevisiae / metabolism
C.elegans tm1200