RRC ID 81131
Author Naka M, Umeno T, Shibuya M, Yamaberi Y, Ueda A, Tanaka M, Takemoto H, Oba M.
Title Intracellular Delivery of Plasmid DNA Using Amphipathic Helical Cell-Penetrating Peptides Containing Dipropylglycine.
Journal Chem Pharm Bull (Tokyo)
Abstract Cell-penetrating peptides (CPPs) serve as potent vehicles for delivering membrane-impermeable compounds, including nucleic acids, into cells. In a previous study, we reported the successful intracellular delivery of small interfering RNAs (siRNAs) with negligible cytotoxicity using a peptide containing an unnatural amino acid (dipropylglycine). In the present study, we employed the same seven peptides as the previous study to evaluate their efficacy in delivering plasmid DNA (pDNA) intracellularly. Although pDNA and siRNA are nucleic acids, they differ in size and biological function, which may influence the optimal peptide sequences for their delivery. Herein, three peptides demonstrated effective pDNA transfection abilities. Notably, only one of the three peptides previously exhibited efficient gene-silencing effect with siRNA. These findings validate our hypothesis and offer insights for the personalized design of CPPs for the delivery of pDNA and siRNA.
Volume 72(5)
Pages 512-517
Published 2024-1-1
DOI 10.1248/cpb.c24-00221
PMID 38811213
MeSH Cell Survival / drug effects Cell-Penetrating Peptides* / chemistry DNA* / chemistry Glycine / chemistry HeLa Cells Humans Plasmids* RNA, Small Interfering* / administration & dosage RNA, Small Interfering* / chemistry Transfection
Resource
DNA material pCAcc-Luc+ (RDB02443)