RRC ID |
81131
|
Author |
Naka M, Umeno T, Shibuya M, Yamaberi Y, Ueda A, Tanaka M, Takemoto H, Oba M.
|
Title |
Intracellular Delivery of Plasmid DNA Using Amphipathic Helical Cell-Penetrating Peptides Containing Dipropylglycine.
|
Journal |
Chem Pharm Bull (Tokyo)
|
Abstract |
Cell-penetrating peptides (CPPs) serve as potent vehicles for delivering membrane-impermeable compounds, including nucleic acids, into cells. In a previous study, we reported the successful intracellular delivery of small interfering RNAs (siRNAs) with negligible cytotoxicity using a peptide containing an unnatural amino acid (dipropylglycine). In the present study, we employed the same seven peptides as the previous study to evaluate their efficacy in delivering plasmid DNA (pDNA) intracellularly. Although pDNA and siRNA are nucleic acids, they differ in size and biological function, which may influence the optimal peptide sequences for their delivery. Herein, three peptides demonstrated effective pDNA transfection abilities. Notably, only one of the three peptides previously exhibited efficient gene-silencing effect with siRNA. These findings validate our hypothesis and offer insights for the personalized design of CPPs for the delivery of pDNA and siRNA.
|
Volume |
72(5)
|
Pages |
512-517
|
Published |
2024-1-1
|
DOI |
10.1248/cpb.c24-00221
|
PMID |
38811213
|
MeSH |
Cell Survival / drug effects
Cell-Penetrating Peptides* / chemistry
DNA* / chemistry
Glycine / chemistry
HeLa Cells
Humans
Plasmids*
RNA, Small Interfering* / administration & dosage
RNA, Small Interfering* / chemistry
Transfection
|
Resource |
DNA material |
pCAcc-Luc+ (RDB02443) |