| RRC ID |
81241
|
| 著者 |
Lee D, Jo MG, Min KY, Choi MY, Kim YM, Kim HS, Choi WS.
|
| タイトル |
IL-10+ regulatory B cells mitigate atopic dermatitis by suppressing eosinophil activation.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Atopic dermatitis (AD) presents significant therapeutic challenges due to its poorly understood etiology. Eosinophilia, a hallmark of allergic inflammation, is implicated in AD pathogenesis. Interleukin-10 (IL-10)-producing regulatory B (Breg) cells exhibit potent anti-inflammatory effects. However, their role in controlling AD-related eosinophilia is not well understood. To investigate the impact of eosinophils on AD, we employed IL-5Rα-deficient (Il5ra-/-) mice, which lack functional eosinophils. Induction of AD in these mice resulted in attenuated disease symptoms, underscoring the critical role of eosinophils in AD development. Additionally, the adoptive transfer of purified Breg cells into mice with AD significantly alleviated disease severity. Mechanistic studies revealed that IL-10 produced by Breg cells directly inhibits eosinophil activation and infiltration into the skin. In vitro experiments further confirmed that Breg cells inhibited eosinophil peroxidase secretion in an IL-10-dependent manner. Our collective findings demonstrate that IL-10 from Breg cells alleviates AD by suppressing eosinophil activation and tissue infiltration. This study elucidates a novel regulatory mechanism of Breg cells, providing a foundation for future Breg-mediated therapeutic strategies for AD.
|
| 巻・号 |
14(1)
|
| ページ |
18164
|
| 公開日 |
2024-8-6
|
| DOI |
10.1038/s41598-024-68660-9
|
| PII |
10.1038/s41598-024-68660-9
|
| PMID |
39107352
|
| PMC |
PMC11303538
|
| MeSH |
Adoptive Transfer
Animals
B-Lymphocytes, Regulatory* / immunology
B-Lymphocytes, Regulatory* / metabolism
Dermatitis, Atopic* / immunology
Dermatitis, Atopic* / metabolism
Dermatitis, Atopic* / pathology
Dermatitis, Atopic* / therapy
Disease Models, Animal
Eosinophils* / immunology
Eosinophils* / metabolism
Interleukin-10* / metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Skin / immunology
Skin / metabolism
Skin / pathology
|
| IF |
3.998
|
| リソース情報 |
| 実験動物マウス |
RBRC00995 |
