RRC ID 81247
Author Tal I, Zhang Y, Jørgensen ME, Pisanty O, Barbosa IC, Zourelidou M, Regnault T, Crocoll C, Olsen CE, Weinstain R, Schwechheimer C, Halkier BA, Nour-Eldin HH, Estelle M, Shani E.
Title The Arabidopsis NPF3 protein is a GA transporter.
Journal Nat Commun
Abstract Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.
Volume 7
Pages 11486
Published 2016-5-3
DOI 10.1038/ncomms11486
PII ncomms11486
PMID 27139299
PMC PMC4857387
MeSH Abscisic Acid / metabolism Abscisic Acid / pharmacology Arabidopsis / drug effects* Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism Biological Transport Carrier Proteins / genetics* Carrier Proteins / metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant* Gibberellins / metabolism Gibberellins / pharmacology* Luminescent Proteins / genetics Luminescent Proteins / metabolism Mutation Plant Growth Regulators / metabolism Plant Growth Regulators / pharmacology Plant Roots / drug effects Plant Roots / genetics Plant Roots / growth & development Plant Roots / metabolism Plants, Genetically Modified Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Seeds / drug effects Seeds / genetics Seeds / growth & development Seeds / metabolism Signal Transduction
Resource
Arabidopsis / Cultured plant cells, genes pda08234