Reference - Detail
| RRC ID | 81330 |
|---|---|
| Author | Ni L, Yamada T, Nakatani K. |
| Title | Utility of oligonucleotide in upregulating circular RNA production in a cellular model. |
| Journal | Sci Rep |
| Abstract |
Circular RNAs (circRNAs), are a covalently closed, single-stranded RNA without 5'- and 3'-termini, commonly stem from the exons of precursor mRNAs (pre-mRNAs). They have recently garnered interest, with studies uncovering their pivotal roles in regulating various aspects of cell functions and disease progressions. A notable feature of circRNA lies in the mechanism of its biogenesis involving a specialized form of splicing: back-splicing. A splicing process that relies on interactions between introns flanking the circularizing exon to bring the up and downstream splice sites in proximity through the formation of a prerequisite hairpin structure, allowing the spliceosomes to join the two splice sites together to produce a circular RNA molecule. Based on this mechanism, we explored the feasibility of facilitating the formation of such a prerequisite hairpin structure by utilizing a newly designed oligonucleotide, CircuLarIzation Promoting OligoNucleotide (CLIP-ON), to promote the production of circRNA in cells. CLIP-ON was designed to hybridize with and physically bridge two distal sequences in the flanking introns of the circularizing exons. The feasibility of CLIP-ON was confirmed in HeLa cells using a model pre-mRNA, demonstrating the applicability of CLIP-ON as a trans-acting modulator to upregulate the production of circRNAs in a cellular environment. |
| Volume | 14(1) |
| Pages | 8096 |
| Published | 2024-4-6 |
| DOI | 10.1038/s41598-024-58663-x |
| PII | 10.1038/s41598-024-58663-x |
| PMID | 38582789 |
| PMC | PMC10998836 |
| MeSH | HeLa Cells Humans RNA* / genetics RNA* / metabolism RNA Precursors / metabolism RNA Splicing / genetics RNA, Circular* / genetics |
| IF | 3.998 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |