論文 - 詳細
| RRC ID | 81357 |
|---|---|
| 著者 | Burkhard SB, Bakkers J. |
| タイトル | Spatially resolved RNA-sequencing of the embryonic heart identifies a role for Wnt/β-catenin signaling in autonomic control of heart rate. |
| ジャーナル | Elife |
| Abstract |
Development of specialized cells and structures in the heart is regulated by spatially -restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, combining high-throughput RNA-sequencing with tissue-sectioning, to establish a genome-wide expression dataset with high spatial resolution for the developing zebrafish heart. Analysis of the dataset revealed over 1100 genes differentially expressed in sub-compartments. Pacemaker cells in the sinoatrial region induce heart contractions, but little is known about the mechanisms underlying their development. Using our transcriptome map, we identified spatially restricted Wnt/β-catenin signaling activity in pacemaker cells, which was controlled by Islet-1 activity. Moreover, Wnt/β-catenin signaling controls heart rate by regulating pacemaker cellular response to parasympathetic stimuli. Thus, this high-resolution transcriptome map incorporating all cell types in the embryonic heart can expose spatially restricted molecular pathways critical for specific cardiac functions. |
| 巻・号 | 7 |
| 公開日 | 2018-2-5 |
| DOI | 10.7554/eLife.31515 |
| PII | 31515 |
| PMID | 29400650 |
| PMC | PMC5815850 |
| MeSH | Animals Heart / embryology* Heart / physiology* Heart Rate* High-Throughput Nucleotide Sequencing Spatio-Temporal Analysis Wnt Signaling Pathway* Zebrafish Zebrafish Proteins / metabolism* beta Catenin / metabolism* |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 38 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |