RRC ID 81362
Author Bremer J, Granato M.
Title Myosin phosphatase Fine-tunes Zebrafish Motoneuron Position during Axonogenesis.
Journal PLoS Genet
Abstract During embryogenesis the spinal cord shifts position along the anterior-posterior axis relative to adjacent tissues. How motor neurons whose cell bodies are located in the spinal cord while their axons reside in adjacent tissues compensate for such tissue shift is not well understood. Using live cell imaging in zebrafish, we show that as motor axons exit from the spinal cord and extend through extracellular matrix produced by adjacent notochord cells, these cells shift several cell diameters caudally. Despite this pronounced shift, individual motoneuron cell bodies stay aligned with their extending axons. We find that this alignment requires myosin phosphatase activity within motoneurons, and that mutations in the myosin phosphatase subunit mypt1 increase myosin phosphorylation causing a displacement between motoneuron cell bodies and their axons. Thus, we demonstrate that spinal motoneurons fine-tune their position during axonogenesis and we identify the myosin II regulatory network as a key regulator.
Volume 12(11)
Pages e1006440
Published 2016-11-1
DOI 10.1371/journal.pgen.1006440
PII PGENETICS-D-16-00502
PMID 27855159
PMC PMC5147773
MeSH Animals Axons* Embryonic Development / genetics* Gene Expression Regulation, Developmental Motor Neurons / cytology Motor Neurons / metabolism Muscle Cells / cytology Muscle Cells / metabolism Myosin-Light-Chain Phosphatase / biosynthesis Myosin-Light-Chain Phosphatase / genetics* Neurogenesis / genetics* Notochord / growth & development Spinal Cord / growth & development Zebrafish / genetics Zebrafish / growth & development
Resource
Zebrafish Evx1 UAS:GFP