RRC ID 81482
Author Yuan D, Zhang X, Yang Y, Wei L, Li H, Zhao T, Guo M, Li Z, Huang Z, Wang M, Dai Z, Li P, Xia Q, Qian W, Cheng D.
Title Schlank orchestrates insect developmental transition by switching H3K27 acetylation to trimethylation in the prothoracic gland.
Journal Proc Natl Acad Sci U S A
Abstract Insect developmental transitions are precisely coordinated by ecdysone and juvenile hormone (JH). We previously revealed that accumulated H3K27 trimethylation (H3K27me3) at the locus encoding JH signal transducer Hairy is involved in the larval-pupal transition in insects, but the underlying mechanism remains to be fully defined. Here, we show in Drosophila and Bombyx that Rpd3-mediated H3K27 deacetylation in the prothoracic gland during the last larval instar promotes ecdysone biosynthesis and the larval-pupal transition by enabling H3K27me3 accumulation at the Hairy locus to induce its transcriptional repression. Importantly, we find that the homeodomain transcription factor Schlank acts to switch active H3K27 acetylation (H3K27ac) to repressive H3K27me3 at the Hairy locus by directly binding to the Hairy promoter and then recruiting the histone deacetylase Rpd3 and the histone methyltransferase PRC2 component Su(z)12 through physical interactions. Moreover, Schlank inhibits Hairy transcription to facilitate the larval-pupal transition, and the Schlank signaling cascade is suppressed by JH but regulated in a positive feedback manner by ecdysone. Together, our data uncover that Schlank mediates epigenetic reprogramming of H3K27 modifications in hormone actions during insect developmental transition.
Volume 121(35)
Pages e2401861121
Published 2024-8-27
DOI 10.1073/pnas.2401861121
PMID 39167603
MeSH Acetylation Animals Basic Helix-Loop-Helix Transcription Factors Bombyx / genetics Bombyx / growth & development Bombyx / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Ecdysone* / metabolism Gene Expression Regulation, Developmental* Histone Deacetylases / genetics Histone Deacetylases / metabolism Histones* / metabolism Insect Proteins / genetics Insect Proteins / metabolism Juvenile Hormones / metabolism Larva* / genetics Larva* / growth & development Larva* / metabolism Methylation Pupa / genetics Pupa / growth & development Pupa / metabolism Repressor Proteins Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism
Resource
Drosophila DGRC#120052 HMC03086