論文 - 詳細
| RRC ID | 81726 |
|---|---|
| 著者 | Chen JJ, Moy C, Pagé V, Monnin C, El-Hajj ZW, Avizonis DZ, Reyes-Lamothe R, Tanny JC. |
| タイトル | The Rtf1/Prf1-dependent histone modification axis counteracts multi-drug resistance in fission yeast. |
| ジャーナル | Life Sci Alliance |
| Abstract |
RNA polymerase II transcription elongation directs an intricate pattern of histone modifications. This pattern includes a regulatory cascade initiated by the elongation factor Rtf1, leading to monoubiquitylation of histone H2B, and subsequent methylation of histone H3 on lysine 4. Previous studies have defined the molecular basis for these regulatory relationships, but it remains unclear how they regulate gene expression. To address this question, we investigated a drug resistance phenotype that characterizes defects in this axis in the model eukaryote Schizosaccharomyces pombe (fission yeast). The mutations caused resistance to the ribonucleotide reductase inhibitor hydroxyurea (HU) that correlated with a reduced effect of HU on dNTP pools, reduced requirement for the S-phase checkpoint, and blunting of the transcriptional response to HU treatment. Mutations in the C-terminal repeat domain of the RNA polymerase II large subunit Rpb1 led to similar phenotypes. Moreover, all the HU-resistant mutants also exhibited resistance to several azole-class antifungal agents. Our results suggest a novel, shared gene regulatory function of the Rtf1-H2Bub1-H3K4me axis and the Rpb1 C-terminal repeat domain in controlling fungal drug tolerance. |
| 巻・号 | 7(6) |
| 公開日 | 2024-6-1 |
| DOI | 10.26508/lsa.202302494 |
| PII | 7/6/e202302494 |
| PMID | 38514187 |
| PMC | PMC10958104 |
| MeSH | Drug Resistance, Multiple Histone Code Histones / genetics Histones / metabolism RNA Polymerase II / genetics RNA Polymerase II / metabolism Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism |
| IF | 2.622 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY32725 FY7448 FY7811 |