| RRC ID |
81775
|
| Author |
Hisayoshi Nozaki, Ryo Matsuzaki, Koichi Shimotori, Noriko Ueki, Wirawan Heman, Wuttipong Mahakham, Haruyo Yamaguchi, Yuuhiko Tanabe, Masanobu Kawachi
|
| Title |
Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa
|
| Journal |
PLOS ONE
|
| Abstract |
Volvox sect. Volvox is a group of green algae with unique morphological features (thick cytoplasmic bridges between somatic cells and spiny zygote walls) and a worldwide distribution. Despite research interest in the diversity of organisms in ancient lakes, Volvox sect. Volvox from ancient lakes worldwide has not been identified to the species level. Here, we established clonal cultures of two species of this group originating from Lake Biwa, an ancient lake in Japan, and performed identification based on morphological and molecular data. One was identified as Volvox kirkiorum based on the nuclear ribosomal DNA internal spacer region (ITS) sequence, bisexual (monoicous or monoecious) spheroids, and zygote morphology. The other showed genetic separation from related species based on the secondary structure of the ITS and results of phylogenetic analysis of a combined data set from the nuclear actin gene, ITS, and two plastid genes (large subunit of RuBisCO and photosystem II CP43 apoprotein gene); it represented a new phylogenetic lineage within Volvox sect. Volvox, suggesting possible endemism in Lake Biwa. This species produced bisexual spheroids with different zygote morphology and zygote number from other species with bisexual spheroids in Volvox sect. Volvox. Therefore, Volvox biwakoensis Nozaki et H. Yamaguchi sp. nov. is described herein. This is the first endemic species of the genus Volvox described from an ancient lake.
|
| Volume |
19
|
| Pages |
e0310549
|
| Published |
2024-9-23
|
| DOI |
10.1371/journal.pone.0310549
|
| PMID |
39312548
|
| PMC |
PMC11419359
|
| MeSH |
DNA, Ribosomal Spacer / genetics
East Asian People
Japan
Lakes*
Phylogeny*
Volvox* / classification
Volvox* / cytology
Volvox* / genetics
|
| Resource |
| Algae |
NIES-543
NIES-2736
NIES-2740
NIES-3874
NIES-4021
NIES-4022
NIES-4258
NIES-4259
NIES-4336
NIES-4337
NIES-4434 |
