RRC ID 81812
Author Tanaka J, Miwa Y, Miyoshi K, Ueno A, Inoue H.
Title Construction of Epstein-Barr virus-based expression vector containing mini-oriP.
Journal Biochem Biophys Res Commun
Abstract Epstein-Barr virus (EBV)-based vectors are extrachromosomal vectors carrying a replicational origin, oriP (about 2200 bp) and a replication initiation factor (EBNA-1) which are sufficient for autonomous replication. Because one disadvantage of these vectors is their large sizes, we examined the effect of partial deletion of oriP on the effectiveness of the EBV-based vectors, using an enhanced green fluorescent protein (EGFP) as a reporter to monitor gene expression. Results indicated that 954 bp-deleted mini-oriP is useful in primate cells since the vector showed high efficiency of stable transfection, a high ratio of EGFP-positive cells, and high recovery of intact plasmid DNA from transfected cells.
Volume 264(3)
Pages 938-43
Published 1999-11-2
DOI 10.1006/bbrc.1999.1617
PII S0006-291X(99)91617-0
PMID 10544034
MeSH Animals Cells, Cultured Epidermal Growth Factor / genetics Gene Transfer Techniques* Genes, Reporter Genetic Engineering Genetic Therapy Genetic Vectors* Herpesvirus 4, Human* Humans Mice Rats Replication Origin / genetics*
Resource
DNA material pEB6CAGMCS Amp/Puro (RDB20292) pEB6CAGMCS Kan/Neo (RDB20293) pEB6CAGMCS SRZeo (RDB20294) pEB6CAGMCS SRHyg (RDB20295) pEB6CAGMCS SVBsd (RDB20296)