論文 - 詳細
| RRC ID | 81812 |
|---|---|
| 著者 | Tanaka J, Miwa Y, Miyoshi K, Ueno A, Inoue H. |
| タイトル | Construction of Epstein-Barr virus-based expression vector containing mini-oriP. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Epstein-Barr virus (EBV)-based vectors are extrachromosomal vectors carrying a replicational origin, oriP (about 2200 bp) and a replication initiation factor (EBNA-1) which are sufficient for autonomous replication. Because one disadvantage of these vectors is their large sizes, we examined the effect of partial deletion of oriP on the effectiveness of the EBV-based vectors, using an enhanced green fluorescent protein (EGFP) as a reporter to monitor gene expression. Results indicated that 954 bp-deleted mini-oriP is useful in primate cells since the vector showed high efficiency of stable transfection, a high ratio of EGFP-positive cells, and high recovery of intact plasmid DNA from transfected cells. |
| 巻・号 | 264(3) |
| ページ | 938-43 |
| 公開日 | 1999-11-2 |
| DOI | 10.1006/bbrc.1999.1617 |
| PII | S0006-291X(99)91617-0 |
| PMID | 10544034 |
| MeSH | Animals Cells, Cultured Epidermal Growth Factor / genetics Gene Transfer Techniques* Genes, Reporter Genetic Engineering Genetic Therapy Genetic Vectors* Herpesvirus 4, Human* Humans Mice Rats Replication Origin / genetics* |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pEB6CAGMCS Amp/Puro (RDB20292) pEB6CAGMCS Kan/Neo (RDB20293) pEB6CAGMCS SRZeo (RDB20294) pEB6CAGMCS SRHyg (RDB20295) pEB6CAGMCS SVBsd (RDB20296) |