| RRC ID |
81812
|
| Author |
Tanaka J, Miwa Y, Miyoshi K, Ueno A, Inoue H.
|
| Title |
Construction of Epstein-Barr virus-based expression vector containing mini-oriP.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Epstein-Barr virus (EBV)-based vectors are extrachromosomal vectors carrying a replicational origin, oriP (about 2200 bp) and a replication initiation factor (EBNA-1) which are sufficient for autonomous replication. Because one disadvantage of these vectors is their large sizes, we examined the effect of partial deletion of oriP on the effectiveness of the EBV-based vectors, using an enhanced green fluorescent protein (EGFP) as a reporter to monitor gene expression. Results indicated that 954 bp-deleted mini-oriP is useful in primate cells since the vector showed high efficiency of stable transfection, a high ratio of EGFP-positive cells, and high recovery of intact plasmid DNA from transfected cells.
|
| Volume |
264(3)
|
| Pages |
938-43
|
| Published |
1999-11-2
|
| DOI |
10.1006/bbrc.1999.1617
|
| PII |
S0006-291X(99)91617-0
|
| PMID |
10544034
|
| MeSH |
Animals
Cells, Cultured
Epidermal Growth Factor / genetics
Gene Transfer Techniques*
Genes, Reporter
Genetic Engineering
Genetic Therapy
Genetic Vectors*
Herpesvirus 4, Human*
Humans
Mice
Rats
Replication Origin / genetics*
|
| Resource |
| DNA material |
pEB6CAGMCS Amp/Puro (RDB20292)
pEB6CAGMCS Kan/Neo (RDB20293)
pEB6CAGMCS SRZeo (RDB20294)
pEB6CAGMCS SRHyg (RDB20295)
pEB6CAGMCS SVBsd (RDB20296) |
