| RRC ID |
81823
|
| 著者 |
Abé T, Yamazaki M, Nozumi M, Maruyama S, Takamura K, Ohashi R, Ajioka Y, Tanuma JI.
|
| タイトル |
Ladinin-1 in actin arcs of oral squamous cell carcinoma is involved in cell migration and epithelial phenotype.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Histopathologically, oral squamous cell carcinoma (OSCC) consists of well-defined interfaces with adjacent non-cancerous epithelium. Previously, we found that SCC tissues expressed higher levels of specific proteins at this interface. Ladinin-1 (LAD1) is one of the specific molecules that has increased expressions in cancer fronts; however, its function in OSCC is unknown. Therefore, this study aimed to elucidate the function of LAD1 in human OSCC cells. LAD1 was localized on the actin arc at the distal periphery of cell clusters in the OSCC cell lines HSC-2, HSC-3, and HSC-4. When LAD1 was knocked down, cellular migration was repressed in wound scratch assays but was reversed in three-dimensional collagen gel invasion assays. Characteristic LAD1 localization along actin arcs forming the leading edge of migrating cells was diminished with loss of filopodia formation and ruffling in knockdown cells, in which the expression levels of cell motility-related genes-p21-activated kinase 1 (PAK1) and caveolin-1 (CAV1)-were upregulated and downregulated, respectively. LAD1 expression was also associated with the downregulation of vimentin and increased histological differentiation of OSCC. These results suggest that LAD1 is involved in actin dynamics during filopodia and lamellipodia formation, and in maintaining the epithelial phenotype of OSCC cells.
|
| 巻・号 |
14(1)
|
| ページ |
22778
|
| 公開日 |
2024-10-1
|
| DOI |
10.1038/s41598-024-74041-z
|
| PII |
10.1038/s41598-024-74041-z
|
| PMID |
39354061
|
| PMC |
PMC11445451
|
| MeSH |
Actins* / metabolism
Carcinoma, Squamous Cell* / genetics
Carcinoma, Squamous Cell* / metabolism
Carcinoma, Squamous Cell* / pathology
Caveolin 1 / genetics
Caveolin 1 / metabolism
Cell Line, Tumor
Cell Movement*
Epithelial Cells / metabolism
Epithelial Cells / pathology
Gene Expression Regulation, Neoplastic
Humans
Mouth Neoplasms* / genetics
Mouth Neoplasms* / metabolism
Mouth Neoplasms* / pathology
Phenotype
Vimentin / genetics
Vimentin / metabolism
p21-Activated Kinases / genetics
p21-Activated Kinases / metabolism
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
HSC-2(RCB1945)
HSC-3(RCB1975)
HSC-4(RCB1902) |
