論文 - 詳細
| RRC ID | 81830 |
|---|---|
| 著者 | Masuda S, Kurabayashi N, Nunokawa R, Otobe Y, Kozuka-Hata H, Oyama M, Shibata Y, Inoue JI, Koebis M, Aiba A, Yoshitane H, Fukada Y. |
| タイトル | TRAF7 determines circadian period through ubiquitination and degradation of DBP. |
| ジャーナル | Commun Biol |
| Abstract |
D-site binding protein, DBP, is a clock-controlled transcription factor and drives daily rhythms of physiological processes through the regulation of an array of genes harboring a DNA binding motif, D-box. DBP protein levels show a circadian oscillation with an extremely robust peak/trough ratio, but it is elusive how the temporal pattern is regulated by post-translational regulation. In this study, we show that DBP protein levels are down-regulated by the ubiquitin-proteasome pathway. Analysis using 19 dominant-negative forms of E2 enzymes have revealed that UBE2G1 and UBE2T mediate the degradation of DBP. A proteomic analysis of DBP-interacting proteins and database screening have identified Tumor necrosis factor Receptor-Associated Factor 7 (TRAF7), a RING-type E3 ligase, that forms a complex with UBE2G1 and/or UBE2T. Ubiquitination analysis have revealed that TRAF7 enhances K48-linked polyubiquitination of DBP in cultured cells. Overexpression of TRAF7 down-regulates DBP protein level, while knockdown of TRAF7 up-regulates DBP in cultured cells. Knockout of TRAF7 in NIH3T3 cells have revealed that TRAF7 mediates the time-of-the-day-dependent regulation of DBP levels. Furthermore, TRAF7 has a period-shortening effect on the cellular clock. Together, TRAF7 plays an important role in circadian clock oscillation through destabilization of DBP. |
| 巻・号 | 7(1) |
| ページ | 1280 |
| 公開日 | 2024-10-8 |
| DOI | 10.1038/s42003-024-07002-x |
| PII | 10.1038/s42003-024-07002-x |
| PMID | 39379486 |
| PMC | PMC11461874 |
| MeSH | Animals Circadian Clocks / genetics Circadian Rhythm* / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Humans Mice NIH 3T3 Cells Proteolysis Transcription Factors / genetics Transcription Factors / metabolism Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / genetics Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / metabolism Ubiquitination* |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3-3-4(RCB1862) |