| RRC ID |
81870
|
| Author |
Miyake T, Tanaka K, Inoue Y, Nagai Y, Nishimura R, Seta T, Nakagawa S, Inoue KI, Hasegawa E, Minamimoto T, Doi M.
|
| Title |
Size-reduced DREADD derivatives for AAV-assisted multimodal chemogenetic control of neuronal activity and behavior.
|
| Journal |
Cell Rep Methods
|
| Abstract |
Designer receptors exclusively activated by designer drugs (DREADDs) are engineered G-protein-coupled receptors that afford reversible manipulation of neuronal activity in vivo. Here, we introduce size-reduced DREADD derivatives miniDq and miniDi, which inherit the basic receptor properties from the Gq-coupled excitatory receptor hM3Dq and the Gi-coupled inhibitory receptor hM4Di, respectively, while being approximately 30% smaller in size. Taking advantage of the compact size of the receptors, we generated an adeno-associated virus (AAV) vector carrying both miniDq and the other DREADD family receptor (κ-opioid receptor-based inhibitory DREADD [KORD]) within the maximum AAV capacity (4.7 kb), allowing us to modulate neuronal activity and animal behavior in both excitatory and inhibitory directions using a single viral vector. We confirmed that expressing miniDq, but not miniDi, allowed activation of striatum activity in the cynomolgus monkey (Macaca fascicularis). The compact DREADDs may thus widen the opportunity for multiplexed interrogation and/or intervention in neuronal regulation in mice and non-human primates.
|
| Volume |
4(10)
|
| Pages |
100881
|
| Published |
2024-10-21
|
| DOI |
10.1016/j.crmeth.2024.100881
|
| PII |
S2667-2375(24)00264-9
|
| PMID |
39437713
|
| MeSH |
Animals
Behavior, Animal / drug effects
Clozapine / analogs & derivatives
Clozapine / pharmacology
Dependovirus* / genetics
Designer Drugs* / chemistry
Designer Drugs* / pharmacology
Genetic Vectors*
HEK293 Cells
Humans
Macaca fascicularis*
Male
Mice
Neurons* / drug effects
Neurons* / metabolism
Receptors, Opioid, kappa / genetics
Receptors, Opioid, kappa / metabolism
|
| Resource |
| DNA material |
pAAV-hSyn-miniDq-P2A-mCherry (RDB20117)
pAAV-hSyn-miniDi-P2A-mCherry (RDB20118)
pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD (RDB20119) |
