論文 - 詳細
| RRC ID | 81882 |
|---|---|
| 著者 | Nishida KM, Miyoshi K, Ogino A, Miyoshi T, Siomi H, Siomi MC. |
| タイトル | Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila. |
| ジャーナル | Mol Cell |
| Abstract |
Endogenous small interfering RNAs (endo-siRNAs) in Drosophila are processed by Dicer-2 (Dcr-2) and loaded onto Ago2 by the Dcr-2/R2D2 heterodimer. In r2d2 mutants, the level of endo-siRNAs is unchanged, but endo-siRNAs are misloaded onto Ago1. However, the mechanism underlying the control of endo-siRNA sorting by R2D2 remains unknown. Here, we show that R2D2 controls endo-siRNA sorting by localizing Dcr-2, and presumably endo-siRNA duplexes, to cytoplasmic foci, D2 bodies. Ago2, but not Ago1, localized to D2 bodies. dsRNA-binding-deficient mutant, but not wild-type, R2D2 failed to localize D2 bodies and caused endo-siRNA misdirection to Ago1 in R2D2-depleted cells. However, R2D2 was dispensable for sorting miRNAs and exogenous siRNAs onto Ago1 and Ago2, respectively, in vivo. Endo- and exo-siRNA guide selection also occurred R2D2 independently. The functions of R2D2 are required to avoid endo-siRNA misdirection to Ago1, because Ago1 is capable of loading incompletely complementary miRNA duplexes and endo-siRNA duplexes. |
| 巻・号 | 49(4) |
| ページ | 680-91 |
| 公開日 | 2013-2-21 |
| DOI | 10.1016/j.molcel.2012.12.024 |
| PII | S1097-2765(13)00036-1 |
| PMID | 23375501 |
| MeSH | Animals Argonaute Proteins / metabolism Cell Line Cytoplasmic Granules / metabolism* Drosophila Proteins / chemistry Drosophila Proteins / metabolism Drosophila Proteins / physiology* Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Female Oocytes / metabolism Protein Interaction Domains and Motifs Protein Transport RNA Helicases / chemistry RNA Helicases / metabolism RNA Interference* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* RNA-Binding Proteins / chemistry RNA-Binding Proteins / metabolism RNA-Binding Proteins / physiology* Ribonuclease III / chemistry Ribonuclease III / metabolism |
| IF | 15.584 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 10015Ab-1 |