論文 - 詳細
| RRC ID | 81903 |
|---|---|
| 著者 | Linton C, Wesolowski J, Lobley A, Yamaji T, Hanada K, Paumet F. |
| タイトル | Specialized contact sites regulate the fusion of chlamydial inclusion membranes. |
| ジャーナル | Nat Commun |
| Abstract |
The intracellular bacterial pathogen Chlamydia trachomatis replicates within a membrane-bound compartment called the inclusion. Upon infection with several chlamydiae, each bacterium creates its own inclusion, resulting in multiple inclusions within each host cell. Ultimately, these inclusions fuse together in a process that requires the chlamydial protein IncA. Here, we show that inclusions form unique contact sites (inclusion contact sites, ICSs) prior to fusion, that serve as fusogenic platforms in which specific lipids and chlamydial proteins concentrate. Fusion depends on IncA clustering within ICSs and is regulated by PI(3,4)P2 and sphingolipids. As IncA concentrates within ICSs, its C-terminus likely interacts in trans with IncA on the apposing membrane, securing a high concentration of IncA at fusion sites. This regulatory mechanism contrasts with eukaryotic or viral fusion systems that are either composed of multiple proteins or use a change in pH to initiate membrane fusion. Thus, our study demonstrates that Chlamydia-mediated membrane fusion is primarily regulated by specific structural domains in IncA and its local organization on the inclusion membrane, which is affected by the host cell lipid composition. |
| 巻・号 | 15(1) |
| ページ | 9250 |
| 公開日 | 2024-10-26 |
| DOI | 10.1038/s41467-024-53443-7 |
| PII | 10.1038/s41467-024-53443-7 |
| PMID | 39461996 |
| PMC | PMC11513123 |
| MeSH | Bacterial Proteins* / genetics Bacterial Proteins* / metabolism Chlamydia Infections / metabolism Chlamydia Infections / microbiology Chlamydia trachomatis* / metabolism Chlamydia trachomatis* / physiology HeLa Cells Humans Inclusion Bodies* / metabolism Membrane Fusion* Membrane Proteins Sphingolipids / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) LY-B(RCB1698) LY-B/cLCB1(RCB1699) |