論文 - 詳細
| RRC ID | 81964 |
|---|---|
| 著者 | Nojima H, Kaida A, Matsuya Y, Uo M, Yoshimura RI, Arazi L, Miura M. |
| タイトル | DNA damage response in a 2D-culture model by diffusing alpha-emitters radiation therapy (Alpha-DaRT). |
| ジャーナル | Sci Rep |
| Abstract |
Diffusing alpha-emitters radiation therapy (Alpha-DaRT) is a unique method, in which interstitial sources carrying 224Ra release a chain of short-lived daughter atoms from their surface. Although DNA damage response (DDR) is crucial to inducing cell death after irradiation, how the DDR occurs during Alpha-DaRT treatment has not yet been explored. In this study, we temporo-spatially characterized DDR such as kinetics of DNA double-strand breaks (DSBs) and cell cycle, in two-dimensional (2D) culture conditions qualitatively mimicking Alpha-DaRT treatments, by employing HeLa cells expressing the Fucci cell cycle-visualizing system. The distribution of the alpha-particle pits detected by a plastic nuclear track detector, CR-39, strongly correlated with γH2AX staining, a marker of DSBs, around the 224Ra source, but the area of G2 arrested cells was more widely spread 24 h from the start of the exposure. Thereafter, close time-lapse observation revealed varying cell cycle kinetics, depending on the distance from the source. A medium containing daughter nuclides prepared from 224Ra sources allowed us to estimate the radiation dose after 24 h of exposure, and determine surviving fractions. The present experimental model revealed for the first time temporo-spatial information of DDR occurring around the source in its early stages. |
| 巻・号 | 14(1) |
| ページ | 11468 |
| 公開日 | 2024-5-20 |
| DOI | 10.1038/s41598-024-62071-6 |
| PII | 10.1038/s41598-024-62071-6 |
| PMID | 38769339 |
| PMC | PMC11106084 |
| MeSH | Alpha Particles* Cell Culture Techniques / methods Cell Cycle / radiation effects DNA Breaks, Double-Stranded* / radiation effects DNA Damage / radiation effects HeLa Cells Histones / metabolism Humans |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.2 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa/Fucci(SA)5#16(RCB4917) |