| RRC ID |
82047
|
| Author |
Kikuchi T, Matsuura K, Shimizu T.
|
| Title |
Low-adhesion culture selection for human iPS cell-derived cardiomyocytes.
|
| Journal |
Sci Rep
|
| Abstract |
Despite progress in generating cardiomyocytes from pluripotent stem cells, these populations often include non-contractile cells, necessitating cardiomyocyte selection for experimental purpose. This study explores a novel cardiomyocyte enrichment mechanism: low-adhesion culture selection. The cardiac cells derived from human induced pluripotent stem cells were subjected to a coating-free low-adhesion culture using bovine serum albumin and high molecular weight dextran sulfate. This approach effectively increased the population of cardiac troponin T-positive cardiomyocytes. Similar results were obtained with commercially available low-adhesion culture dishes. Subsequently, we accessed the practicality of selection of cardiomyocytes using this phenomenon by comparing it with established methods such as glucose-free culture and selection based on puromycin resistance genes. The cardiomyocytes enriched through low-adhesion culture selection maintained autonomous pulsation and responsiveness to beta-stimuli. Moreover, no significant differences were observed in the expression of genes related to subtype commitment and maturation when compared to other selection methods. In conclusion, cardiomyocytes derived from pluripotent stem cells were more low-adhesion culture resistant than their accompanying non-contractile cells, and low-adhesion culture is an alternative method for selection of pluripotent stem cell-derived cardiomyocytes.
|
| Volume |
14(1)
|
| Pages |
11081
|
| Published |
2024-5-15
|
| DOI |
10.1038/s41598-024-60765-5
|
| PII |
10.1038/s41598-024-60765-5
|
| PMID |
38744867
|
| PMC |
PMC11094004
|
| MeSH |
Cell Adhesion*
Cell Culture Techniques* / methods
Cell Differentiation*
Cells, Cultured
Humans
Induced Pluripotent Stem Cells* / cytology
Induced Pluripotent Stem Cells* / metabolism
Myocytes, Cardiac* / cytology
Myocytes, Cardiac* / metabolism
Troponin T / genetics
Troponin T / metabolism
|
| Resource |
| Human and Animal Cells |
201B7(HPS0063) |
