論文 - 詳細
| RRC ID | 82051 |
|---|---|
| 著者 | Urasawa T, Koizumi T, Kimura K, Ohta Y, Kawasaki N. |
| タイトル | Quantitative Proteomics for the Development and Manufacturing of Human-Induced Pluripotent Stem Cell-Derived Neural Stem Cells Using Data-Independent Acquisition Mass Spectrometry. |
| ジャーナル | J Proteome Res |
| Abstract |
Human-induced pluripotent stem cell (iPSC)-derived neural stem cells (NSCs) have several potential applications in regenerative medicine. A deep understanding of stem cell characteristics is critical for developing appropriate products for use in the clinic. This study aimed to develop approaches for characterizing iPSC-derived NSCs. Data-independent acquisition mass spectrometry (DIA-MS) was used to obtain temporal proteomic profiles of differentiating cells. Principal component analysis of the proteome profiles allowed for the discrimination of cells cultured for different periods. Cells were characterized by Gene Ontology analysis to annotate the upregulated proteins based on their functions. We found that trophoblast glycoprotein (TPBG), a membrane glycoprotein that inhibits the Wnt/β-catenin pathway, was elevated in NSC and that silencing TPBG promoted proliferation rather than neuronal differentiation. Treatment with Wnt/β-catenin pathway activators and inhibitors showed that modulating the Wnt/β-catenin pathway is crucial for differentiation into NSC. These results suggest that the level of TPBG is critical for differentiation into NSC, and TPBG is a potentially critical quality attribute of differentiating cells. In summary, DIA-MS-based proteomics is a promising multi-attribute method for characterizing stem cell-derived products. |
| 巻・号 | 22(6) |
| ページ | 1843-1854 |
| 公開日 | 2023-6-2 |
| DOI | 10.1021/acs.jproteome.2c00841 |
| PMID | 37097202 |
| PMC | PMC10243303 |
| MeSH | Cell Differentiation Humans Induced Pluripotent Stem Cells* / metabolism Neural Stem Cells* Proteomics Wnt Signaling Pathway beta Catenin / genetics beta Catenin / metabolism |
| IF | 4.074 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 610B1(HPS0331) |