| RRC ID |
82135
|
| 著者 |
Chow T, Humble W, Lucarelli E, Onofrillo C, Choong PF, Di Bella C, Duchi S.
|
| タイトル |
Feasibility and barriers to rapid establishment of patient-derived primary osteosarcoma cell lines in clinical management.
|
| ジャーナル |
iScience
|
| Abstract |
Osteosarcoma is a highly aggressive primary bone tumor that has seen little improvement in survival rates in the past three decades. Preclinical studies are conducted on a small pool of commercial cell lines which may not fully reflect the genetic heterogeneity of this complex cancer, potentially hindering translatability of in vitro results. Developing a single-site laboratory protocol to rapidly establish patient-derived primary cancer cell lines (PCCL) within a clinically actionable time frame of a few weeks will have significant scientific and clinical ramifications. These PCCL can widen the pool of available cell lines for study while patient-specific data could derive therapeutic correlation. This endeavor is exceedingly challenging considering the proposed time constraints. By proposing key definitions and a clear theoretical framework, this evaluation of osteosarcoma cell line establishment methodology over the past three decades assesses feasibility by identifying barriers and suggesting solutions, thereby facilitating systematic experimentation and optimization.
|
| 巻・号 |
27(9)
|
| ページ |
110251
|
| 公開日 |
2024-9-20
|
| DOI |
10.1016/j.isci.2024.110251
|
| PII |
S2589-0042(24)01476-7
|
| PMID |
39286504
|
| PMC |
PMC11403063
|
| IF |
4.447
|
| リソース情報 |
| ヒト・動物細胞 |
NOS-1(RCB1032)
NOS-2(RCB1033)
NOS-10(RCB2348) |
