論文 - 詳細
| RRC ID | 82138 |
|---|---|
| 著者 | Kai R, Hatakeyama M, Iwamoto S, Kitaoka T. |
| タイトル | Primary human mesenchymal stem cell culture under xeno-free conditions using surface-modified cellulose nanofiber scaffolds. |
| ジャーナル | Carbohydr Polym |
| Abstract |
Stem cell culture often requires various animal-derived components such as serum and collagen. This limits its practical use. Therefore, xeno-free (xenogeneic component-free) culture systems are receiving increased attention. Herein, we propose xeno-free, plant-derived cellulose nanofibers (CNFs) with different surface chemistry: 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized CNFs (TOCNFs) with carboxy groups and surface-sulfated CNFs (S-CNFs) for the proliferation of human mesenchymal stem cells (hMSCs) under various serum conditions. We cultured bone marrow-derived hMSCs on CNF scaffolds with various fiber lengths and functional group contents. Original CNFs were bioinert materials that did not contribute to cell adhesion. In contrast, the surface-modified CNFs facilitated the proliferation of immortalized hMSCs under normal and low-serum conditions. The TOCNFs (COONa: 1.47 mmol g-1; length: 0.53 μm), the S-CNFs (OSO3Na: 0.64 mmol g-1; 0.61 μm), and a combination of the two (1:1 by weight) enabled immortalized hMSCs to maintain their multipotency, even under serum-free conditions. Primary cultured hMSCs proliferated well on the TOCNF/S-CNF scaffolds in a completely serum-free medium, comparable to animal-derived type I collagen, although few hMSCs adhered to the standard polystyrene substrate. Our strategy of using surface-modified CNFs will inform the development of xeno-free culture systems to avoid the use of animal-derived materials for both cell culture media and scaffolds. |
| 巻・号 | 343 |
| ページ | 122479 |
| 公開日 | 2024-11-1 |
| DOI | 10.1016/j.carbpol.2024.122479 |
| PII | S0144-8617(24)00705-7 |
| PMID | 39174138 |
| MeSH | Cell Adhesion / drug effects Cell Culture Techniques / methods Cell Differentiation / drug effects Cell Proliferation* / drug effects Cells, Cultured Cellulose* / chemistry Cyclic N-Oxides / chemistry Humans Mesenchymal Stem Cells* / cytology Mesenchymal Stem Cells* / drug effects Nanofibers* / chemistry Surface Properties Tissue Scaffolds* / chemistry |
| IF | 7.182 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MSC-R37(HMS0020) MSC-R50(HMS0047) |