Reference - Detail
| RRC ID | 82154 |
|---|---|
| Author | Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T. |
| Title | Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons. |
| Journal | STAR Protoc |
| Abstract |
cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize Pcdh trans-interactions across cells as a preliminary experiment before using dissociated mouse neurons. We then detail procedures for visualizing Pcdh trans-interactions between processes originating from the same neurons, which are considered as Pcdh-mediated neuronal self-recognition. For complete details on the use and execution of this protocol, please refer to Kanadome et al.1. |
| Volume | 5(1) |
| Pages | 102844 |
| Published | 2024-3-15 |
| DOI | 10.1016/j.xpro.2024.102844 |
| PII | S2666-1667(24)00009-1 |
| PMID | 38277267 |
| PMC | PMC10840325 |
| MeSH | Animals Cadherins* / genetics Cadherins* / metabolism Cell Adhesion HEK293 Cells Humans Mice Neurons / metabolism Protocadherins* |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) |