RRC ID 82474
Author Tang J, Nakamura M, Ng WY, Feng N, Ueno M.
Title Novel pof1 mutation suppresses the sensitivity to DNA replication inhibitor in fission yeast RecQ helicase mutant.
Journal Biochem Biophys Res Commun
Abstract Homologous recombination is vital for DNA double-strand break repair. Dysfunction in homologous recombination can lead to cell death, mutations, and cancer. In fission yeast (Schizosaccharomyces pombe), RecQ helicase Rqh1 resolves recombination intermediates. We found that rqh1-hd strain impaired growth in media containing hydroxyurea and thiabendazole. Using this condition, we identified a novel pof1 mutation (pof1-A81T) that suppress the poor growth of the rqh1-hd strain on the plate containing hydroxyurea and thiabendazole. Compared to rqh1-hd, rqh1-hd pof1-A81T cells displayed reduced Replication Protein A foci on chromosome bridges after hydroxyurea treatment. This suggests that pof1-A81T mutation suppresses the accumulation of recombination intermediates in hydroxyurea-treated rqh1-hd cells. Additionally, pof1-A81T mutation rescued the segregation defect of nucleolar protein Gar2 observed in hydroxyurea-treated rqh1-hd cells, potentially by mitigating recombination intermediate accumulation in rDNA. These results suggest that the pof1-A81T mutation suppresses the accumulation of recombination intermediates, particularly in rDNA, and alleviates the rqh1 deficiency phenotype in S. pombe.
Volume 741
Pages 151014
Published 2024-12-31
DOI 10.1016/j.bbrc.2024.151014
PII S0006-291X(24)01550-X
PMID 39580958
MeSH DNA Helicases DNA Replication* / drug effects Homologous Recombination / drug effects Hydroxyurea* / pharmacology Mutation* Nucleic Acid Synthesis Inhibitors / pharmacology RecQ Helicases* / genetics RecQ Helicases* / metabolism Schizosaccharomyces* / drug effects Schizosaccharomyces* / genetics Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism Thiabendazole / pharmacology
Resource
Yeast FY14970