RRC ID 82516
Author Xiang Y, Liu S, Wan S, Chen Q, Song Y, Feng G, Zhang X, Bai L, Zhu Y.
Title Interleukin-1 increases SERPINE1 expression in human granulosa-lutein cell via P50/P52 signaling pathways.
Journal Mol Cell Endocrinol
Abstract It has been reported that immune factors are associated with the occurrence of polycystic ovary syndrome (PCOS). Interleukin-1 (IL-1) is a member of the interleukin family that widely participates in the regulation of the inflammatory response in the immune system. In addition, it has been reported that aberrant IL-1 accumulation in serum is associated with the occurrence of PCOS. However, little is known about how IL-1 participates in the pathogenesis of PCOS. In the present study, we demonstrated that the immune microenvironment was altered in follicular fluid from PCOS patients and that the expression levels of two IL-1 cytokines, IL-1α and IL-1β were increased. Transcriptome analysis revealed that IL-1α and IL-1β treatment induced primary human granulosa-lutein (hGL) cell inflammatory response and increased the expression of serpin family E member 1 (SERPINE1). Mechanistically, we demonstrated that IL-1α and IL-1β upregulated SERPINE1 expression through IL-1R1-mediated activation of downstream P50 and P52 signaling pathways in human granulosa cells. Our study highlighted the role of immune state changes in the occurrence of PCOS and provided new insight into the treatment of patients with IL-1-induced ovarian function disorders.
Volume 591
Pages 112274
Published 2024-9-15
DOI 10.1016/j.mce.2024.112274
PII S0303-7207(24)00130-8
PMID 38777211
MeSH Adult Cells, Cultured Female Follicular Fluid / metabolism Gene Expression Regulation / drug effects Granulosa Cells* / drug effects Granulosa Cells* / metabolism Humans Interleukin-1* / genetics Interleukin-1* / metabolism Interleukin-1alpha / genetics Interleukin-1alpha / metabolism Interleukin-1beta / metabolism Luteal Cells* / drug effects Luteal Cells* / metabolism Plasminogen Activator Inhibitor 1* / genetics Plasminogen Activator Inhibitor 1* / metabolism Polycystic Ovary Syndrome* / genetics Polycystic Ovary Syndrome* / metabolism Receptors, Interleukin-1 Type I / genetics Receptors, Interleukin-1 Type I / metabolism Signal Transduction*
Resource
Human and Animal Cells KGN(RCB1154)