論文 - 詳細
| RRC ID | 82583 |
|---|---|
| 著者 | Logsdon DM, Ming H, Ezashi T, West RC, Schoolcraft WB, Roberts RM, Jiang Z, Yuan Y. |
| タイトル | Transcriptome comparisons of trophoblasts from regenerative cell models with peri-implantation human embryos†. |
| ジャーナル | Biol Reprod |
| Abstract |
Mechanisms controlling trophoblast (TB) proliferation and differentiation during embryo implantation are poorly understood. Human trophoblast stem cells (TSC) and BMP4/A83-01/PD173074-treated pluripotent stem cell-derived trophoblast cells (BAP) are two widely employed, contemporary models to study TB development and function, but how faithfully they mimic early TB cells has not been fully examined. We evaluated the transcriptomes of TB cells from BAP and TSC and directly compared them with those from peri-implantation human embryos during extended embryo culture (EEC) between embryonic days 8 to 12. The BAP and TSC grouped closely with TB cells from EEC within each TB sublineage following dimensional analysis and unsupervised hierarchical clustering. However, subtle differences in transcriptional programs existed within each TB sublineage. We also validated the presence of six genes in peri-implantation human embryos by immunolocalization. Our analysis reveals that both BAP and TSC models have features of peri-implantation TB s, while maintaining minor transcriptomic differences, and thus serve as valuable tools for studying implantation in lieu of human embryos. |
| 巻・号 | 111(5) |
| ページ | 1000-1016 |
| 公開日 | 2024-11-11 |
| DOI | 10.1093/biolre/ioae120 |
| PII | 7728794 |
| PMID | 39109839 |
| MeSH | Cell Differentiation Embryo Culture Techniques Embryo Implantation* / physiology Embryo, Mammalian / metabolism Female Gene Expression Regulation, Developmental Humans Transcriptome* Trophoblasts* / metabolism |
| IF | 3.322 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CT27(RCB4936) CT29(RCB4937) |