RRC ID 82634
Author Shibata R, Konishi H, Arita T, Yamamoto Y, Matsuda H, Yamamoto T, Ohashi T, Shimizu H, Komatsu S, Shiozaki A, Kubota T, Fujiwara H, Otsuji E.
Title Extracellular glypican-1 affects tumor progression and prognosis in esophageal cancer.
Journal Cancer Med
Abstract INTRODUCTION:Cells are covered with a glycan surface layer that is referred to as the glycocalyx (GCX). It has been reported that the formation of the GCX is promoted on cancer cells and is associated with tumor growth and metastasis. Heparan sulfate proteoglycan glypican-1 (GPC1) is a core protein of the GCX that is overexpressed in esophageal squamous cell carcinoma (ESCC) and is involved in the development and progression of cancer cells. The purpose of the present study is to analyze the utility of GPC1 as a new biomarker ralated to glycocalyx that reflects therapeutic effect and prognosis of ESCC.
METHODS:We measured the concentration of GPC1 protein in preoperative plasma from advanced esophageal cancer patients and examined its relationships with clinicopathological factors and therapeutic efficacy, and the effects of extracellular GPC1 were investigated.
RESULTS:The following clinical factors were significantly correlated with the preoperative high GPC1 concentration: male, tumor size ≥30 mm, venous invasion, pT factor ≥2, pStage ≥3, residual tumor, and distant metastatic recurrence. Both overall and recurrence-free survival were significantly worse in the high GPC1 group. Extracellular GPC1 protein concentration reflected intracellular GPC1 expression. Furthermore, we examined the effects of extracellular recombinant human (rh)GPC1 on ESCC cells, and found that extracellular rhGPC1 affects cell motility, including migration and invasion.
CONCLUSIONS:These results demonstrated the utility of extracellular GPC1 as a biomarker, which can be assayed from a less invasive blood sample-based liquid biopsy. Extracellular GPC1 protein plays a role in both tumor cell motility and cancer progression. Thus, plasma GPC1 is a useful biomarker for esophageal cancer progression and may be a potential candidate of therapeutic target.
Volume 13(18)
Pages e70212
Published 2024-9-1
DOI 10.1002/cam4.70212
PMID 39300946
PMC PMC11413415
MeSH Aged Biomarkers, Tumor* / metabolism Cell Line, Tumor Cell Movement Disease Progression* Esophageal Neoplasms* / blood Esophageal Neoplasms* / genetics Esophageal Neoplasms* / metabolism Esophageal Neoplasms* / mortality Esophageal Neoplasms* / pathology Esophageal Squamous Cell Carcinoma / blood Esophageal Squamous Cell Carcinoma / genetics Esophageal Squamous Cell Carcinoma / metabolism Esophageal Squamous Cell Carcinoma / mortality Esophageal Squamous Cell Carcinoma / pathology Esophageal Squamous Cell Carcinoma / surgery Female Glypicans* / blood Glypicans* / genetics Glypicans* / metabolism Humans Male Middle Aged Neoplasm Staging Prognosis
Resource
Human and Animal Cells TE-5(RCB1949) TE-8(RCB2098) TE-11(RCB2100)