RRC ID 82636
Author Iwasaki T, Maruyama A, Inui Y, Sakurai T, Kawano T.
Title In vitro transcytosis of Helicobacter pylori histidine-rich protein through gastric epithelial-like cells and the blood-brain barrier.
Journal Biosci Biotechnol Biochem
Abstract Recent epidemiological studies have supported the correlation between Helicobacter pylori infection and the development of Alzheimer's disease. HpHpn, a histidine-rich H. pylori protein, forms amyloid-like oligomers; it may be a pathogenic factor for Alzheimer's disease progression. HpHpn may also be transported from the gastric epithelium to the brain. However, HpHpn is secreted from H. pylori on the outer surface of gastric epithelia; therefore, the hypothesized movement of HpHpn across the gastric epithelium to the blood remains controversial. Here, we found the HpHpn showed acidic pH-dependent cellular uptake and subsequent secretion in human gastric epithelial-like carcinoma cells. Furthermore, HpHpn exhibited in vitro permeability across the blood-brain barrier. Although further in vivo experiments are required, our findings suggest that in vitro transcytosis of HpHpn in gastric epithelial cells and the blood-brain barrier may provide new insights into the correlation between H. pylori infections and Alzheimer's disease progression.
Volume 86(3)
Pages 321-330
Published 2022-2-24
DOI 10.1093/bbb/zbab221
PII 6479114
PMID 34935901
MeSH Alzheimer Disease / metabolism Alzheimer Disease / microbiology Bacterial Proteins* / metabolism Blood-Brain Barrier* / metabolism Blood-Brain Barrier* / microbiology Cell Line, Tumor Epithelial Cells* / metabolism Epithelial Cells* / microbiology Gastric Mucosa / metabolism Gastric Mucosa / microbiology Helicobacter Infections / metabolism Helicobacter Infections / microbiology Helicobacter pylori* / metabolism Humans Hydrogen-Ion Concentration Proteins Transcytosis*
Resource
Human and Animal Cells HGC-27(RCB0500) MKN74(RCB1002)