Reference - Detail
| RRC ID | 82691 |
|---|---|
| Author | Takeuchi K, Tabe S, Yamamoto Y, Takahashi K, Matsuo M, Ueno Y, Ohtsuka M, Morinaga S, Miyagi Y, Yamaguchi T, Tanimizu N, Taniguchi H. |
| Title | Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment. |
| Journal | STAR Protoc |
| Abstract |
Pancreatic ductal adenocarcinoma (PDAC) organoids that simulate the tumor microenvironment (TME) are an effective tool to identify how TME affects PDAC malignancy. We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including heterogeneous cancer-associated fibroblasts (CAFs), using patient-derived PDAC cells and human-induced pluripotent cell-derived endothelial and mesenchymal cells. We also describe the procedure for analyzing FPCO characteristics. FPCO can provide a platform for establishing a reliable drug screening system. For complete details on the use and execution of this protocol, please refer to Takeuchi et al.1. |
| Volume | 6(1) |
| Pages | 103539 |
| Published | 2025-1-7 |
| DOI | 10.1016/j.xpro.2024.103539 |
| PII | S2666-1667(24)00704-4 |
| PMID | 39772386 |
| Resource | |
| Human and Animal Cells | 1383D6(HPS1006) 293T(RCB2202) |