論文 - 詳細
| RRC ID | 82740 |
|---|---|
| 著者 | Na EJ, Jeong CG, Chae SB, Oem JK. |
| タイトル | Investigating the reassortment potential and pathogenicity of the S segment in Akabane virus using a reverse genetics system. |
| ジャーナル | BMC Vet Res |
| Abstract |
BACKGROUND:Akabane virus (AKAV) is an arthropod-borne virus that causes congenital malformations and neuropathology in cattle and sheep. In South Korea, AKAVs are classified into two main genogroups: K0505 and AKAV-7 strains. The K0505 strain infects pregnant cattle, leading to fetal abnormalities, while the AKAV-7 strain induces encephalomyelitis in post-natal cattle. The pathogenicities of K0505 and AKAV-7 strains differ significantly; however, the specific gene in the AKAV-7 strain that drives its pathogenicity remains unidentified. In this study, changes in viral replication and pathogenicity were investigated, particularly when the S segment of AKAV-7 was mutated using a T7 RNA polymerase-based reverse genetics (RG) system. RESULTS:The rAKAV-7ΔNSs virus, with a deletion in the NSs protein of the wild-type AKAV-7 virus (wtAKAV-7), and the rAKAV-7(S-K0505) virus, where the S segment of wtAKAV-7 was reassorted with that from the wild type K0505 strain (wtK0505), were successfully rescued. The rAKAV-7ΔNSs virus demonstrated impaired replication in Vero cells and exhibited reduced mortality and RNA viral load in the organs of suckling mice compared to the wtAKAV-7. The rAKAV-7(S-K0505) virus displayed similar growth kinetics in Vero cells and showed no significant reduction in mortality rate in suckling mice compared to wtAKAV-7. CONCLUSIONS:These observations suggest that the S segment, especially the NS protein, is associated with the pathogenicity of AKAV-7. Also, the results imply that the L and M segments might explain the differences in pathogenicity between the AKAV-7 and K0505 strains. Moreover, our findings indicate the potential for reassortment between distinct genogroups of AKAVs. |
| 巻・号 | 21(1) |
| ページ | 20 |
| 公開日 | 2025-1-15 |
| DOI | 10.1186/s12917-024-04459-0 |
| PII | 10.1186/s12917-024-04459-0 |
| PMID | 39815297 |
| PMC | PMC11734451 |
| MeSH | Animals Bunyaviridae Infections* / veterinary Bunyaviridae Infections* / virology Cattle Cattle Diseases / virology Chlorocebus aethiops Mice Orthobunyavirus* / genetics Orthobunyavirus* / pathogenicity Reassortant Viruses* / genetics Reassortant Viruses* / pathogenicity Reverse Genetics* Sheep Vero Cells Virulence Virus Replication |
| IF | 1.835 |
| リソース情報 | |
| ヒト・動物細胞 | BHK/T7-9(RCB4942) |