論文 - 詳細
| RRC ID | 82789 |
|---|---|
| 著者 | Akerberg AA, Stewart S, Stankunas K. |
| タイトル | Spatial and temporal control of transgene expression in zebrafish. |
| ジャーナル | PLoS One |
| Abstract |
Transgenic zebrafish research has provided valuable insights into gene functions and cell behaviors directing vertebrate development, physiology, and disease models. Most approaches use constitutive transgene expression and therefore do not provide control over the timing or levels of transgene induction. We describe an inducible gene expression system that uses new tissue-specific zebrafish transgenic lines that express the Gal4 transcription factor fused to the estrogen-binding domain of the human estrogen receptor. We show these Gal4-ERT driver lines confer rapid, tissue-specific induction of UAS-controlled transgenes following tamoxifen exposure in both embryos and adult fish. We demonstrate how this technology can be used to define developmental windows of gene function by spatiotemporal-controlled expression of constitutively active Notch1 in embryos. Given the array of existing UAS lines, the modular nature of this system will enable many previously intractable zebrafish experiments. |
| 巻・号 | 9(3) |
| ページ | e92217 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0092217 |
| PII | PONE-D-13-46380 |
| PMID | 24643048 |
| PMC | PMC3958484 |
| MeSH | Animals Animals, Genetically Modified DNA-Binding Proteins / genetics Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Organ Specificity Peptide Elongation Factor 1 / genetics Promoter Regions, Genetic Rabbits Receptor, Notch1 / biosynthesis Receptor, Notch1 / genetics Receptors, Estrogen / biosynthesis Receptors, Estrogen / genetics Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / genetics* Tamoxifen / pharmacology Transcription Factors / genetics Transcriptional Activation / drug effects* Transgenes Xenopus Proteins / genetics Xenopus laevis / genetics Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / genetics beta-Globins / genetics |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |