| RRC ID |
82789
|
| 著者 |
Akerberg AA, Stewart S, Stankunas K.
|
| タイトル |
Spatial and temporal control of transgene expression in zebrafish.
|
| ジャーナル |
PLoS One
|
| Abstract |
Transgenic zebrafish research has provided valuable insights into gene functions and cell behaviors directing vertebrate development, physiology, and disease models. Most approaches use constitutive transgene expression and therefore do not provide control over the timing or levels of transgene induction. We describe an inducible gene expression system that uses new tissue-specific zebrafish transgenic lines that express the Gal4 transcription factor fused to the estrogen-binding domain of the human estrogen receptor. We show these Gal4-ERT driver lines confer rapid, tissue-specific induction of UAS-controlled transgenes following tamoxifen exposure in both embryos and adult fish. We demonstrate how this technology can be used to define developmental windows of gene function by spatiotemporal-controlled expression of constitutively active Notch1 in embryos. Given the array of existing UAS lines, the modular nature of this system will enable many previously intractable zebrafish experiments.
|
| 巻・号 |
9(3)
|
| ページ |
e92217
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0092217
|
| PII |
PONE-D-13-46380
|
| PMID |
24643048
|
| PMC |
PMC3958484
|
| MeSH |
Animals
Animals, Genetically Modified
DNA-Binding Proteins / genetics
Green Fluorescent Proteins / biosynthesis
Green Fluorescent Proteins / genetics
Organ Specificity
Peptide Elongation Factor 1 / genetics
Promoter Regions, Genetic
Rabbits
Receptor, Notch1 / biosynthesis
Receptor, Notch1 / genetics
Receptors, Estrogen / biosynthesis
Receptors, Estrogen / genetics
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / genetics*
Tamoxifen / pharmacology
Transcription Factors / genetics
Transcriptional Activation / drug effects*
Transgenes
Xenopus Proteins / genetics
Xenopus laevis / genetics
Zebrafish / genetics*
Zebrafish / metabolism
Zebrafish Proteins / genetics
beta-Globins / genetics
|
| IF |
2.74
|
| リソース情報 |
| ゼブラフィッシュ |
UAS:GFP |
