論文 - 詳細
| RRC ID | 82793 |
|---|---|
| 著者 | Sato Y, Mukai M, Ueda J, Muraki M, Stasevich TJ, Horikoshi N, Kujirai T, Kita H, Kimura T, Hira S, Okada Y, Hayashi-Takanaka Y, Obuse C, Kurumizaka H, Kawahara A, Yamagata K, Nozaki N, Kimura H. |
| タイトル | Genetically encoded system to track histone modification in vivo. |
| ジャーナル | Sci Rep |
| Abstract |
Post-translational histone modifications play key roles in gene regulation, development, and differentiation, but their dynamics in living organisms remain almost completely unknown. To address this problem, we developed a genetically encoded system for tracking histone modifications by generating fluorescent modification-specific intracellular antibodies (mintbodies) that can be expressed in vivo. To demonstrate, an H3 lysine 9 acetylation specific mintbody (H3K9ac-mintbody) was engineered and stably expressed in human cells. In good agreement with the localization of its target acetylation, H3K9ac-mintbody was enriched in euchromatin, and its kinetics measurably changed upon treatment with a histone deacetylase inhibitor. We also generated transgenic fruit fly and zebrafish stably expressing H3K9ac-mintbody for in vivo tracking. Dramatic changes in H3K9ac-mintbody localization during Drosophila embryogenesis could highlight enhanced acetylation at the start of zygotic transcription around mitotic cycle 7. Together, this work demonstrates the broad potential of mintbody and lays the foundation for epigenetic analysis in vivo. |
| 巻・号 | 3 |
| ページ | 2436 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/srep02436 |
| PII | srep02436 |
| PMID | 23942372 |
| PMC | PMC3743053 |
| MeSH | Acetylation Amino Acid Sequence Animals Cell Line Drosophila Proteins / metabolism Drosophila melanogaster / embryology Drosophila melanogaster / metabolism Embryonic Development Genetic Techniques* Green Fluorescent Proteins / metabolism Histones / metabolism* Humans Intracellular Space / metabolism Lysine / metabolism Mice Molecular Sequence Data Protein Processing, Post-Translational* Single-Chain Antibodies / metabolism Zebrafish |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | SAGFF(LF)73A |