論文 - 詳細
| RRC ID | 82816 |
|---|---|
| 著者 | Hattori M, Wazawa T, Orioka M, Hiruta Y, Nagai T. |
| タイトル | Creating coveted bioluminescence colors for simultaneous multi-color bioimaging. |
| ジャーナル | Sci Adv |
| Abstract |
Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of the bioluminescent proteins must sufficiently vary to enable simultaneous detection. Here, we aimed to introduce a method that can be used to expand the color variation by tuning dual-acceptor bioluminescence resonance energy transfer. Using this approach, we could visualize multiple targets with up to 20 colors through single-shot acquisition using a color complementary metal-oxide semiconductor camera. Overall, this method enables simple and simultaneous observation of multiple biological targets and phenomena. |
| 巻・号 | 11(4) |
| ページ | eadp4750 |
| 公開日 | 2025-1-24 |
| DOI | 10.1126/sciadv.adp4750 |
| PMID | 39841832 |
| PMC | PMC11753369 |
| MeSH | Animals Color* Humans Luminescent Measurements* / methods Luminescent Proteins / chemistry Luminescent Proteins / metabolism |
| IF | 13.117 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 132 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pcDNA3 Venus-Akaluc (RDB15781) |
| ヒト・動物細胞 | C2C12(RCB0987) 293(RCB1637) HeLa |