Reference - Detail
| RRC ID | 83082 |
|---|---|
| Author | Kato Y, Mioka T, Uemura S, Abe F. |
| Title | Role of a novel endoplasmic reticulum-resident glycoprotein Mtc6/Ehg2 in high-pressure growth: stability of tryptophan permease Tat2 in Saccharomyces cerevisiae. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Deep-sea organisms are subjected to extreme conditions; therefore, understanding their adaptive strategies is crucial. We utilize Saccharomyces cerevisiae as a model to investigate pressure-dependent protein regulation and piezo-adaptation. Using yeast deletion library analysis, we identified 6 poorly characterized genes that are crucial for high-pressure growth, forming novel functional modules associated with cell growth. In this study, we aimed to unravel the molecular mechanisms of high-pressure adaptation in S. cerevisiae, focusing on the role of MTC6. MTC6, the gene encoding the novel glycoprotein Mtc6/Ehg2, was found to stabilize tryptophan permease Tat2, ensuring efficient tryptophan uptake and growth under high pressure at 25 MPa. The loss of MTC6 led to promoted vacuolar degradation of Tat2, depending on the Rsp5-Bul1 ubiquitin ligase complex. These findings enhance our understanding of deep-sea adaptations and stress biology, with broad implications for biotechnology, environmental microbiology, and evolutionary insights across species. |
| Volume | 88(9) |
| Pages | 1055-1063 |
| Published | 2024-8-26 |
| DOI | 10.1093/bbb/zbae086 |
| PII | 7699096 |
| PMID | 38918055 |
| MeSH | Amino Acid Transport Systems* / genetics Amino Acid Transport Systems* / metabolism Endoplasmic Reticulum* / metabolism Endosomal Sorting Complexes Required for Transport / genetics Endosomal Sorting Complexes Required for Transport / metabolism Hydrostatic Pressure Protein Stability Proteolysis Saccharomyces cerevisiae* / genetics Saccharomyces cerevisiae* / metabolism Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism Tryptophan* / metabolism Ubiquitin-Protein Ligase Complexes / genetics Ubiquitin-Protein Ligase Complexes / metabolism Vacuoles / metabolism |
| IF | 1.516 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | BYP9806 |