| RRC ID |
83316
|
| 著者 |
Ishikawa H, Kuno Y, Yokoo T, Nagashima R, Takaki T, Sasaki H, Kohda C, Iyoda M.
|
| タイトル |
In vitro investigation of the effects of Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 exopolysaccharides on tight junction damage caused by influenza virus infection.
|
| ジャーナル |
Lett Appl Microbiol
|
| Abstract |
It is a problem that influenza virus infection increases susceptibility to secondary bacterial infection in lungs leading to lethal pneumonia. We previously reported that exopolysaccharides (EPS) derived from Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (OLL1073R-1) could prevent against influenza virus infection followed by secondary bacterial infection in vitro. Therefore, the present study assessed whether EPS derived OLL1073R-1 protects the alveolar epithelial barrier disfunction caused by influenza virus infection. After A549 cells treated with EPS or without EPS were infected influenza virus A/Puerto Rico/8/34 (IFV) for 12 h, the levels of tight junction genes expression and inflammatory genes expression were measured by reverse transcription polymerase chain reaction. As results, EPS treatment could protect against low-titer IFV infection, but not high-titer IFV infection, followed by suppression of the increased expression of inflammatory cytokine gene levels and recovery of the decrease in the expression level of ZO-1 gene that was caused by low-titer IFV infection, leading to an improvement trend in the barrier function. Our findings showed that EPS derived from OLL1073R-1 could inhibit low-titer IFV infection leading to maintenance of the epithelial barrier function through the suppression of inflammatory cytokine genes expression.
|
| 巻・号 |
77(4)
|
| 公開日 |
2024-4-8
|
| DOI |
10.1093/lambio/ovae029
|
| PII |
7634354
|
| PMID |
38521981
|
| MeSH |
Bacterial Infections*
Cytokines / genetics
Cytokines / metabolism
Humans
Influenza, Human*
Lactobacillus delbrueckii* / genetics
Lactobacillus delbrueckii* / metabolism
Orthomyxoviridae*
Tight Junctions
|
| IF |
2.173
|
| リソース情報 |
| ヒト・動物細胞 |
A549 |
