Reference - Detail
| RRC ID | 83324 |
|---|---|
| Author | Hirono N, Hashimoto M, Shimojo H, Sasaki H. |
| Title | Fate specification triggers a positive feedback loop of TEAD-YAP and NANOG to promote epiblast formation in preimplantation embryos. |
| Journal | Development |
| Abstract |
In preimplantation embryos, epiblast (EPI) fate specification from the inner cell mass is controlled by the segregation of NANOG and GATA6 expression. TEAD-YAP interaction is activated during EPI formation and is required for pluripotency factor expression. These events occur asynchronously with similar timing during EPI formation, and their relationship remains elusive. Here, we examined the relationship between NANOG-GATA6 and TEAD-YAP. The nuclear accumulation of YAP takes place only in EPI-specified cells, and a positive feedback loop operates between NANOG and TEAD-YAP. The effects of TEAD-YAP on SOX2 upregulation in EPI-specified cells are likely indirect. EPI fate specification also alters the response of Nanog, Sox2 and Cdx2 to TEAD-YAP. These results suggest that EPI-fate specification alters the transcriptional network from the morula-like to the EPI-specified state and activates TEAD-YAP to trigger a positive feedback loop with NANOG, which stabilizes the EPI fate. The coordinated occurrence of these processes in individual cells likely supports proper EPI formation under the condition of asynchronous EPI-fate specification. |
| Volume | 152(1) |
| Published | 2025-1-1 |
| DOI | 10.1242/dev.203091 |
| PII | 363294 |
| PMID | 39629521 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Animals Blastocyst* / cytology Blastocyst* / metabolism CDX2 Transcription Factor / genetics CDX2 Transcription Factor / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Differentiation DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Feedback, Physiological* Female GATA6 Transcription Factor / genetics GATA6 Transcription Factor / metabolism Gene Expression Regulation, Developmental* Germ Layers* / cytology Germ Layers* / metabolism Mice Nanog Homeobox Protein* / genetics Nanog Homeobox Protein* / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / metabolism TEA Domain Transcription Factors / metabolism Transcription Factors* / genetics Transcription Factors* / metabolism YAP-Signaling Proteins* / metabolism |
| IF | 5.611 |
| Resource | |
| DNA material | pT2K-BI-TRE-HA-Lats2/EGFP (RDB20709) |