Reference - Detail
| RRC ID | 83354 |
|---|---|
| Author | Itaya M, Fujita K, Kuroki A, Tsuge K. |
| Title | Bottom-up genome assembly using the Bacillus subtilis genome vector. |
| Journal | Nat Methods |
| Abstract |
We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences. |
| Volume | 5(1) |
| Pages | 41-3 |
| Published | 2008-1-1 |
| DOI | 10.1038/nmeth1143 |
| PII | nmeth1143 |
| PMID | 18066072 |
| MeSH | Bacillus subtilis / genetics* Bacterial Proteins / genetics* DNA, Bacterial / genetics* Genetic Engineering / methods* Genetic Vectors / genetics* Genome, Bacterial / genetics* Recombinant Proteins / genetics* |
| IF | 30.822 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 16 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes B. subtilis | MBP34 MBP35 |