論文 - 詳細
| RRC ID | 83416 |
|---|---|
| 著者 | Igaki T, Suzuki Y, Tokushige N, Aonuma H, Takahashi R, Miura M. |
| タイトル | Evolution of mitochondrial cell death pathway: Proapoptotic role of HtrA2/Omi in Drosophila. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Despite the essential role of mitochondria in a variety of mammalian cell death processes, the involvement of mitochondrial pathway in Drosophila cell death has remained unclear. To address this, we cloned and characterized DmHtrA2, a Drosophila homolog of a mitochondrial serine protease HtrA2/Omi. We show that DmHtrA2 normally resides in mitochondria and is up-regulated by UV-irradiation. Upon receipt of apoptotic stimuli, DmHtrA2 is translocated to extramitochondrial compartment; however, unlike its mammalian counterpart, the extramitochondrial DmHtrA2 does not diffuse throughout the cytosol but stays near the mitochondria. RNAi-mediated knock-down of DmHtrA2 in larvae or adult flies results in a resistance to stress stimuli. DmHtrA2 specifically cleaves Drosophila inhibitor-of-apoptosis protein 1 (DIAP1), a cellular caspase inhibitor, and induces cell death both in vitro and in vivo as potent as other fly cell death proteins. Our observations suggest that DmHtrA2 promotes cell death through a cleavage of DIAP1 in the vicinity of mitochondria, which may represent a prototype of mitochondrial cell death pathway in evolution. |
| 巻・号 | 356(4) |
| ページ | 993-7 |
| 公開日 | 2007-5-18 |
| DOI | 10.1016/j.bbrc.2007.03.079 |
| PII | S0006-291X(07)00573-6 |
| PMID | 17397804 |
| MeSH | Animals Apoptosis / physiology* Apoptosis Regulatory Proteins / metabolism* Cells, Cultured Drosophila Drosophila Proteins / metabolism* High-Temperature Requirement A Serine Peptidase 2 Inhibitor of Apoptosis Proteins / metabolism* Mitochondria / physiology* Mitochondria / ultrastructure Mitochondrial Proteins / metabolism* Serine Endopeptidases / metabolism* Signal Transduction / physiology |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#119589 |