| RRC ID |
83492
|
| 著者 |
Guo Y, Kitano T, Inoue K, Murano K, Hirose M, Li TD, Sakashita A, Ishizu H, Ogonuki N, Matoba S, Sato M, Ogura A, Siomi H.
|
| タイトル |
Obox4 promotes zygotic genome activation upon loss of Dux.
|
| ジャーナル |
Elife
|
| Abstract |
Once fertilized, mouse zygotes rapidly proceed to zygotic genome activation (ZGA), during which long terminal repeats (LTRs) of murine endogenous retroviruses with leucine tRNA primer (MERVL) are activated by a conserved homeodomain-containing transcription factor, DUX. However, Dux-knockout embryos produce fertile mice, suggesting that ZGA is redundantly driven by an unknown factor(s). Here, we present multiple lines of evidence that the multicopy homeobox gene, Obox4, encodes a transcription factor that is highly expressed in mouse two-cell embryos and redundantly drives ZGA. Genome-wide profiling revealed that OBOX4 specifically binds and activates MERVL LTRs as well as a subset of murine endogenous retroviruses with lysine tRNA primer (MERVK) LTRs. Depletion of Obox4 is tolerated by embryogenesis, whereas concomitant Obox4/Dux depletion markedly compromises embryonic development. Our study identified OBOX4 as a transcription factor that provides genetic redundancy to preimplantation development.
|
| 巻・号 |
13
|
| 公開日 |
2024-6-24
|
| DOI |
10.7554/eLife.95856
|
| PII |
95856
|
| PMID |
38856708
|
| PMC |
PMC11196112
|
| MeSH |
Animals
Embryonic Development / genetics
Gene Expression Regulation, Developmental
Genome
Homeodomain Proteins* / genetics
Homeodomain Proteins* / metabolism
Mice
Mice, Knockout
Zygote* / metabolism
|
| IF |
7.08
|
| リソース情報 |
| ヒト・動物細胞 |
EB3(AES0139) |
