Reference - Detail
| RRC ID | 83498 |
|---|---|
| Author | Morikawa K, Nishida H, Imami K, Ishihama Y. |
| Title | One-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography. |
| Journal | Mol Cell Proteomics |
| Abstract |
We have developed a one-step isolation method for protein N-terminal peptides from LysargiNase digests by pipette tip-based strong cation exchange (SCX) chromatography. This CHAMP-N (CHromatographic AMplification of Protein N-terminal peptides) method using disposable and parallel-processable SCX tips instead of conventional HPLC SCX columns facilitates simple, sensitive, reproducible, and high-throughput N-terminomic profiling without sacrificing the high identification numbers and selectivity achieved by the HPLC-based method. By applying the CHAMP-N method to HEK293T cells, we identified novel cleavage sites for signal and transit peptides and non-canonical translation initiation sites. Finally, for proteome-wide terminomics, we present a simple and comprehensive N- and C-terminomics platform employing three different tip-based approaches, including CHAMP-N, in which protease digestion and one-step isolation by tip LC are commonly used to achieve complementary terminome coverages. |
| Volume | 23(9) |
| Pages | 100820 |
| Published | 2024-9-1 |
| DOI | 10.1016/j.mcpro.2024.100820 |
| PII | S1535-9476(24)00110-5 |
| PMID | 39069075 |
| PMC | PMC11382313 |
| MeSH | Chromatography, Ion Exchange / methods HEK293 Cells Humans Peptides* / chemistry Peptides* / metabolism Proteome / metabolism Proteomics* / methods |
| IF | 4.87 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) |