論文 - 詳細
| RRC ID | 83505 |
|---|---|
| 著者 | Miura F, Shibata Y, Miura M, Inatomi K, Suzuki Y, Ito T. |
| タイトル | Identification of an enzyme with strong single-stranded DNA ligation activity and its application for sequencing. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
An enzyme with strong single-stranded DNA (ssDNA) ligation activity would be advantageous for many molecular biology applications. However, currently available enzymes exhibit only limited activity. Here, we identified an enzyme with strong ssDNA ligation activity upon searching the databases for proteins homologous to TS2126 RNA ligase, the known enzyme with the highest yet limited ssDNA ligation activity. A ligase from Thermus phage phiLo (GenBank: AYJ73970) or Tph ssDNA ligase (SDL) depicted higher ssDNA ligation activity than TS2126 RNA ligase. This study suggests that SDL could be useful for various applications including sequencing for library preparation. |
| 巻・号 | 53(3) |
| 公開日 | 2025-1-24 |
| DOI | 10.1093/nar/gkaf054 |
| PII | 7990338 |
| PMID | 39883008 |
| PMC | PMC11780859 |
| MeSH | DNA Ligases* / metabolism DNA, Single-Stranded* / metabolism RNA Ligase (ATP) / chemistry RNA Ligase (ATP) / genetics RNA Ligase (ATP) / metabolism Sequence Analysis, DNA / methods Thermus / enzymology Thermus / genetics Viral Proteins / genetics Viral Proteins / metabolism |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY20134 BY20135 BY20148 BY20149 |