論文 - 詳細
| RRC ID | 83541 |
|---|---|
| 著者 | Sakamoto T. |
| タイトル | Live-Cell Imaging of RNA G-Quadruplex with a Dual-Color Fluorescence Switch-on Probe. |
| ジャーナル | Methods Mol Biol |
| Abstract |
In-cell imaging of the G-quadruplex (G4) formation of nucleic acids remains challenging for revealing G4's functions in cells. This study describes the cell imaging method of G4 nucleic acids using our unique tripodal quinone-cyanine fluorescent dye, QCy(MeBT)3, whose 600 nm and 700 nm fluorescence is enhanced independently upon the binding with double-stranded DNA and G4-DNA/RNA, respectively. The use of QCy(MeBT)3 enables us to visualize cytosolic G4 RNAs in fixed and living HeLa cells with near-infrared 700 nm fluorescence. |
| 巻・号 | 2875 |
| ページ | 83-90 |
| 公開日 | 2025-1-1 |
| DOI | 10.1007/978-1-0716-4248-1_7 |
| PMID | 39535641 |
| MeSH | DNA / chemistry Fluorescent Dyes* / chemistry G-Quadruplexes* HeLa Cells Humans Microscopy, Fluorescence / methods Molecular Imaging / methods Optical Imaging / methods RNA* / chemistry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |