論文 - 詳細
| RRC ID | 83731 |
|---|---|
| 著者 | Chavan A, Isenhart R, Nguyen SC, Kotb NM, Harke J, Sintsova A, Ulukaya G, Uliana F, Ashiono C, Kutay U, Pegoraro G, Rangan P, Joyce EF, Jagannathan M. |
| タイトル | A nuclear architecture screen in Drosophila identifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate. |
| ジャーナル | Genes Dev |
| Abstract |
The association of genomic loci to the nuclear periphery is proposed to facilitate cell type-specific gene repression and influence cell fate decisions. However, the interplay between gene position and expression remains incompletely understood, in part because the proteins that position genomic loci at the nuclear periphery remain unidentified. Here, we used an Oligopaint-based HiDRO screen targeting ∼1000 genes to discover novel regulators of nuclear architecture in Drosophila cells. We identified the heterochromatin-associated protein Stonewall (Stwl) as a factor promoting perinuclear chromatin positioning. In female germline stem cells (GSCs), Stwl binds and positions chromatin loci, including GSC differentiation genes, at the nuclear periphery. Strikingly, Stwl-dependent perinuclear positioning is associated with transcriptional repression, highlighting a likely mechanism for Stwl's known role in GSC maintenance and ovary homeostasis. Thus, our study identifies perinuclear anchors in Drosophila and demonstrates the importance of gene repression at the nuclear periphery for cell fate. |
| 巻・号 | 38(9-10) |
| ページ | 415-435 |
| 公開日 | 2024-6-25 |
| DOI | 10.1101/gad.351424.123 |
| PII | gad.351424.123 |
| PMID | 38866555 |
| PMC | PMC11216176 |
| MeSH | Animals Cell Differentiation* / genetics Cell Nucleus* / genetics Cell Nucleus* / metabolism Chromatin* / genetics Chromatin* / metabolism Drosophila / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Female Gene Expression Regulation, Developmental / genetics Germ Cells / metabolism Stem Cells / cytology Stem Cells / metabolism |
| IF | 9.527 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#141809 |