| RRC ID |
83777
|
| Author |
Ishizaka M, Maeno A, Nakazawa H, Fujii R, Oikawa S, Tani T, Kanno H, Koita R, Kawamura A.
|
| Title |
The functional roles of zebrafish HoxA- and HoxD-related clusters in the pectoral fin development.
|
| Journal |
Sci Rep
|
| Abstract |
The paralogs 9-13 Hox genes in mouse HoxA and HoxD clusters are critical for limb development. When both HoxA and HoxD clusters are deleted in mice, significant limb truncation is observed compared to the phenotypes of single and compound mutants of Hox9-13 genes in these clusters. In zebrafish, mutations in hox13 genes in HoxA- and HoxD-related clusters result in abnormal morphology of pectoral fins, homologous to forelimbs. However, the effect of the simultaneous deletions of entire HoxA- and HoxD-related clusters on pectoral fin development remains unknown. Here, we generated mutants with several combinations of hoxaa, hoxab, and hoxda cluster deletions and analyzed the pectoral fin development. In hoxaa-/-;hoxab-/-;hoxda-/- larvae, the endoskeletal disc and the fin-fold are significantly shortened in developing pectoral fins. In addition, we show that this anomaly is due to defects in the pectoral fin growth after the fin bud formation. Furthermore, in the surviving adult mutants, micro-CT scanning reveals defects in the posterior portion of the pectoral fin which is thought to represent latent regions of the limb. Our results further support that the functional role of HoxA and HoxD clusters is conserved in the paired appendage formation in bony fishes.
|
| Volume |
14(1)
|
| Pages |
23602
|
| Published |
2024-10-9
|
| DOI |
10.1038/s41598-024-74134-9
|
| PII |
10.1038/s41598-024-74134-9
|
| PMID |
39384796
|
| PMC |
PMC11464670
|
| MeSH |
Animal Fins* / growth & development
Animal Fins* / metabolism
Animals
Gene Expression Regulation, Developmental
Homeodomain Proteins* / genetics
Homeodomain Proteins* / metabolism
Multigene Family*
Mutation
Zebrafish* / genetics
Zebrafish Proteins* / genetics
Zebrafish Proteins* / metabolism
|
| IF |
3.998
|
| Resource |
| Zebrafish |
RIKEN WT (RW)
hoxaa cluster sud111
hoxab cluster sud112
hoxda cluster sud116 |
