Reference - Detail
| RRC ID | 83784 |
|---|---|
| Author | Ishii M, Tsurusaki A, Komatsu M, Shiozaki K. |
| Title | Neu1-deficient zebrafish cells exhibit reduced Edwardsiella piscicida infection due to altered lysosomal exocytosis and membrane dynamics. |
| Journal | Fish Shellfish Immunol |
| Abstract |
Edwardsiella piscicida is a Gram-negative intracellular pathogen causing Edwardsiellosis, leading to economic losses in aquaculture. While phagocytosis is its primary infection route, alternative entry pathways remain largely unexplored. Neu1 sialidase, a lysosomal enzyme in glycoconjugate degradation, was investigated for its role in E. piscicida infection using primary cultured cells derived from Neu1-KO zebrafish fin (Neu1-KO cells). Compared to wild-type (WT) cells, Neu1-KO cells exhibited lower infection rates, which were associated with enhanced lysosomal exocytosis. Infection was restored by the intracellular calcium chelator BAPTA-AM, highlighting the role of exocytosis. Leupeptin, a cysteine/serine protease inhibitor, increased E. piscicida infection in Neu1-KO cells. Neu1-KO cells exhibited lower rab10 expression and reduced membrane ruffling, which was restored by BAPTA-AM and leupeptin. Given the role of epidermal growth factor receptor (EGFR) signaling, we assessed its phosphorylation, which was reduced in Neu1-KO cells but restored by treatment with BAPTA-AM and leupeptin. This suggests that inhibiting lysosome exocytosis or extracellular protease activity may enhance EGFR phosphorylation. These findings indicate that the decreased E. piscicida infection in Neu1-KO cells resulted from enhanced lysosomal exocytosis, leading to increased extracellular protease secretion, subsequent EGFR inactivation by extracellular protease, and reduced EGFR-regulated ruffling. This study provides novel insights into the regulatory mechanisms of bacterial infection and lysosomal exocytosis, informing potential therapeutic strategies against intracellular pathogens. |
| Volume | 161 |
| Pages | 110273 |
| Published | 2025-6-1 |
| DOI | 10.1016/j.fsi.2025.110273 |
| PII | S1050-4648(25)00162-7 |
| PMID | 40074189 |
| MeSH | Animals Cells, Cultured Edwardsiella* / physiology Enterobacteriaceae Infections* / genetics Enterobacteriaceae Infections* / immunology Enterobacteriaceae Infections* / microbiology Enterobacteriaceae Infections* / veterinary Exocytosis* Fish Diseases* / immunology Fish Diseases* / microbiology Lysosomes* / physiology Neuraminidase* / genetics Zebrafish* / genetics Zebrafish* / immunology Zebrafish Proteins* / genetics |
| IF | 3.298 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Zebrafish | RIKEN WT (RW) |