| RRC ID |
83844
|
| 著者 |
Wang XL, Wang ZY, Chen XH, Cai Y, Hu B.
|
| タイトル |
Reprogramming miR-146b-snphb Signaling Activates Axonal Mitochondrial Transport in the Zebrafish M-cell and Facilitates Axon Regeneration After Injury.
|
| ジャーナル |
Neurosci Bull
|
| Abstract |
Acute mitochondrial damage and the energy crisis following axonal injury highlight mitochondrial transport as an important target for axonal regeneration. Syntaphilin (Snph), known for its potent mitochondrial anchoring action, has emerged as a significant inhibitor of both mitochondrial transport and axonal regeneration. Therefore, investigating the molecular mechanisms that influence the expression levels of the snph gene can provide a viable strategy to regulate mitochondrial trafficking and enhance axonal regeneration. Here, we reveal the inhibitory effect of microRNA-146b (miR-146b) on the expression of the homologous zebrafish gene syntaphilin b (snphb). Through CRISPR/Cas9 and single-cell electroporation, we elucidated the positive regulatory effect of the miR-146b-snphb axis on Mauthner cell (M-cell) axon regeneration at the global and single-cell levels. Through escape response tests, we show that miR-146b-snphb signaling positively regulates functional recovery after M-cell axon injury. In addition, continuous dynamic imaging in vivo showed that reprogramming miR-146b significantly promotes axonal mitochondrial trafficking in the pre-injury and early stages of regeneration. Our study reveals an intrinsic axonal regeneration regulatory axis that promotes axonal regeneration by reprogramming mitochondrial transport and anchoring. This regulation involves noncoding RNA, and mitochondria-associated genes may provide a potential opportunity for the repair of central nervous system injury.
|
| 巻・号 |
41(4)
|
| ページ |
633-648
|
| 公開日 |
2025-4-1
|
| DOI |
10.1007/s12264-024-01329-5
|
| PII |
10.1007/s12264-024-01329-5
|
| PMID |
39645618
|
| PMC |
PMC11978567
|
| MeSH |
Animals
Axonal Transport* / physiology
Axons* / metabolism
MicroRNAs* / genetics
MicroRNAs* / metabolism
Mitochondria* / metabolism
Nerve Regeneration* / physiology
Nerve Tissue Proteins* / genetics
Nerve Tissue Proteins* / metabolism
Signal Transduction / physiology
Zebrafish
Zebrafish Proteins* / genetics
Zebrafish Proteins* / metabolism
|
| IF |
4.326
|
| リソース情報 |
| ゼブラフィッシュ |
Tol-056 |
