Reference - Detail
| RRC ID | 83979 |
|---|---|
| Author | Waddell BM, Wu CW. |
| Title | A role for the C. elegans Argonaute protein CSR-1 in small nuclear RNA 3' processing. |
| Journal | PLoS Genet |
| Abstract |
The Integrator is a multi-subunit protein complex that catalyzes the maturation of snRNA transcripts via 3' cleavage, a step required for snRNA incorporation with snRNP for spliceosome biogenesis. Here we developed a GFP based in vivo snRNA misprocessing reporter as a readout of Integrator function and performed a genome-wide RNAi screen for Integrator regulators. We found that loss of the Argonaute encoding csr-1 gene resulted in widespread 3' misprocessing of snRNA transcripts that is accompanied by a significant increase in alternative splicing. Loss of the csr-1 gene down-regulates the germline expression of Integrator subunits 4 and 6 and is accompanied by a reduced protein translation efficiency of multiple Integrator catalytic and non-catalytic subunits. Through isoform and motif mutant analysis, we determined that CSR-1's effect on snRNA processing is dependent on its catalytic slicer activity but does not involve the CSR-1a isoform. Moreover, mRNA-sequencing revealed high similarity in the transcriptome profile between csr-1 and Integrator subunit knockdown via RNAi. Together, our findings reveal CSR-1 as a new regulator of the Integrator complex and implicate a novel role of this Argonaute protein in snRNA 3' processing. |
| Volume | 20(5) |
| Pages | e1011284 |
| Published | 2024-5-1 |
| DOI | 10.1371/journal.pgen.1011284 |
| PII | PGENETICS-D-23-01143 |
| PMID | 38743783 |
| PMC | PMC11125478 |
| MeSH | Alternative Splicing / genetics Animals Argonaute Proteins* / genetics Argonaute Proteins* / metabolism Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism RNA Interference RNA Processing, Post-Transcriptional RNA, Small Nuclear* / genetics RNA, Small Nuclear* / metabolism Spliceosomes / genetics Spliceosomes / metabolism |
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| The most frequently cited source | Bluesky |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm892 |