論文 - 詳細
| RRC ID | 84022 |
|---|---|
| 著者 | Lu YM, Yan S, Ti SC, Zheng C. |
| タイトル | Editing of endogenous tubulins reveals varying effects of tubulin posttranslational modifications on axonal growth and regeneration. |
| ジャーナル | Elife |
| Abstract |
Tubulin posttranslational modifications (PTMs) modulate the dynamic properties of microtubules and their interactions with other proteins. However, the effects of tubulin PTMs were often revealed indirectly through the deletion of modifying enzymes or the overexpression of tubulin mutants. In this study, we directly edited the endogenous tubulin loci to install PTM-mimicking or -disabling mutations and studied their effects on microtubule stability, neurite outgrowth, axonal regeneration, cargo transport, and sensory functions in the touch receptor neurons of Caenorhabditis elegans. We found that the status of β-tubulin S172 phosphorylation and K252 acetylation strongly affected microtubule dynamics, neurite growth, and regeneration, whereas α-tubulin K40 acetylation had little influence. Polyglutamylation and detyrosination in the tubulin C-terminal tail had more subtle effects on microtubule stability likely by modulating the interaction with kinesin-13. Overall, our study systematically assessed and compared several tubulin PTMs for their impacts on neuronal differentiation and regeneration and established an in vivo platform to test the function of tubulin PTMs in neurons. |
| 巻・号 | 13 |
| 公開日 | 2024-7-1 |
| DOI | 10.7554/eLife.94583 |
| PII | 94583 |
| PMID | 38949652 |
| PMC | PMC11216746 |
| MeSH | Acetylation Animals Axons / metabolism Axons / physiology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Kinesins / genetics Kinesins / metabolism Microtubules* / metabolism Nerve Regeneration Phosphorylation Protein Processing, Post-Translational* Tubulin* / genetics Tubulin* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3871 tm4059 tm2414 tm683 tm3889 tm3310 tm3360 |